Dehydroascorbate and ascorbate transport in rat liver microsomal vesicles.

The Journal of Biological Chemistry
Gábor BánhegyiAngelo Benedetti

Abstract

Ascorbate and dehydroascorbate transport was investigated in rat liver microsomal vesicles using radiolabeled compounds and a rapid filtration method. The uptake of both compounds was time- and temperature-dependent, and saturable. Ascorbate uptake did not reach complete equilibrium, it had low affinity and high capacity. Ascorbate influx could not be inhibited by glucose, dehydroascorbate, or glucose transport inhibitors (phloretin, cytochalasin B) but it was reduced by the anion transport inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid and by the alkylating agent N-ethylmaleimide. Ascorbate uptake could be stimulated by ferric iron and could be diminished by reducing agents (dithiothreitol, reduced glutathione). In contrast, dehydroascorbate uptake exceeded the level of passive equilibrium, it had high affinity and low capacity. Glucose cis inhibited and trans stimulated the uptake. Glucose transport inhibitors were also effective. The presence of intravesicular reducing compounds increased, while extravesicular reducing environment decreased dehydroascorbate influx. Our results suggest that dehydroascorbate transport is preferred in hepatic endoplasmic reticulum and it is mediated by a GLUT-type transporter. The i...Continue Reading

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Citations

Aug 5, 2003·BioFactors·Jôzsef Mandl, Gábor Bánhegyi
Aug 5, 2003·BioFactors·Miklós CsalaGábor Bánhegyi
Aug 5, 2003·BioFactors·Gábor BánhegyiJózsef Mandl
May 22, 2001·Archives of Biochemistry and Biophysics·M CsalaG Bánhegyi
Sep 21, 2001·Biochemical and Biophysical Research Communications·M CsalaG Bánhegyi
Jul 18, 2001·Pathology Oncology Research : POR·G Bánhegyi, J Mándl
Feb 1, 2003·Archives of Biochemistry and Biophysics·Alexander J MichelsTory M Hagen
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Dec 17, 2009·Antioxidants & Redox Signaling·Miklós CsalaGábor Bánhegyi
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Dec 14, 2011·Antioxidants & Redox Signaling·Miklós CsalaGábor Bánhegyi
Dec 7, 2011·Antioxidants & Redox Signaling·Gábor BánhegyiMiklós Csala
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Jul 14, 2010·FEBS Letters·Eva Margittai, Gábor Bánhegyi
May 1, 2007·FEBS Letters·Gábor BánhegyiJózsef Mandl
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Jul 23, 2008·Journal of Neurochemistry·Gábor BánhegyiMiklós Csala
Jul 17, 2004·Free Radical Biology & Medicine·Yi Long MaKelly L Drew
Jul 19, 2005·Biochemical and Biophysical Research Communications·Gábor NardaiPéter Csermely
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Jun 12, 2008·The Journal of Toxicological Sciences·Masayoshi TakabatakeKunitoshi Mitsumori
Oct 15, 2013·Orvosi hetilap·András Szarka, Tamás Lőrincz
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May 1, 2007·Biochimica Et Biophysica Acta·Miklós CsalaGábor Bánhegyi
Jul 6, 2004·Archives of Biochemistry and Biophysics·András SzarkaHan Asard
Jul 13, 2017·Chemical Reviews·Deborah Fass, Colin Thorpe
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Oct 5, 2017·Antioxidants & Redox Signaling·Szilvia Z TóthAndrás Szarka

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