Dehydroascorbate reductase and monodehydroascorbate reductase activities of two metallothionein-like proteins from sweet potato (Ipomoea batatas [L.] Lam. 'Tainong 57') storage roots

Botanical Studies
Guan-Jhong HuangYaw-Huei Lin

Abstract

Metallothionein (MT) is a group of proteins with low molecular masses and high cysteine contents, and it is classified into different types, which generally contains two domains with typical amino acid sequences. In this report, two full-length cDNAs (MT-1 and MT-II) encoding MT-like proteins were isolated from the roots of sweet potato (Ipomoea batatas [L.] Lam. 'Tainong 57'). Their open reading frames contained 642 and 519 nucleotides (66 and 81 amino acids) for MT-1 and MT-II, respectively, and exhibited a relatively low amino acid sequence similarity. On the basis of the amino acid sequence similarity and conserved residues, it is suggested that MT-I is a member of the plant MT Type-I family, and MT-II is a member of the plant MT Type-II family. The corresponding mRNA levels of MT-1 and MT-II were the highest found in the storage roots. Recombinant MT-1 and MT-II protein overproduced in E. coli (M15) was purified by Ni(2+)-chelated affinity chromatography. MT-1 and MT-II reduced dehydroascorbate (DHA) in the presence of glutathione (GSH) to regenerate L-ascorbic acid (AsA). However, without GSH, MT-1 and MT-II has very low DHA reductase activity. And AsA was oxidized by AsA oxidase to generate monodehydroascorbate (MDA) fre...Continue Reading

References

Sep 12, 2002·Annual Review of Plant Biology·Christopher Cobbett, Peter Goldsbrough
Jul 23, 2003·Current Opinion in Plant Biology·Cristina Pignocchi, Christine H Foyer
Nov 30, 1967·Biochemical and Biophysical Research Communications·J C Kaplan, E Beutler
Sep 28, 2010·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh Gill, Narendra Tuteja
Feb 12, 2011·Plant Biology·V H HassinenS O Kärenlampi
Jun 21, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Eva Freisinger
Sep 11, 2012·Free Radical Biology & Medicine·Patricia I Oteiza
Mar 19, 2013·International Journal of Molecular Sciences·Branislav Ruttkay-NedeckyRene Kizek

❮ Previous
Next ❯

Datasets Mentioned

BETA
AF116845
FJ418632

Methods Mentioned

BETA
AsA
MDA
PCR
X-ray

Software Mentioned

Genetics Computer Group ( GCG )

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.