Dehydroascorbate uptake is impaired in the early response of Arabidopsis plant cell cultures to cadmium

Journal of Experimental Botany
N HoremansYves Guisez

Abstract

The balance between antioxidants, such as ascorbate (ASC) and glutathione, and oxidative reactive oxygen species (ROS) is known to play a pivotal role in the response of plant cells to abiotic stress. Here cell cultures of Arabidopsis thaliana were investigated with regard to their response to elevated levels of cadmium. At concentrations <100 microM, Cd induces a rapid and concentration-dependent H(2)O(2) accumulation. This response could be inhibited by diphenylene iodonium (DPI, 20 microM). Reverse transcription-PCR analysis of three RBOH (respiratory burst oxidase homologues) genes showed an increased transcription of RBOHF after 15 min. No change in ASC concentration was observed during the first 3 h after Cd addition. In contrast, glutathione levels completely diminished within 1 h. This drop could be attributed to an increase in phytochelatin 4. At the plasma membrane, Cd further induced a significant decrease in dehydroascorbate (DHA) uptake activity (up to 90% inhibition after 4 h). This decrease is not present when cells are treated with LaCl(3) before exposure to CdCl(2). LaCl(3) is a typical inhibitor of Ca channels and prevents Cd uptake in these cells as well as the Cd-induced ROS production. Therefore, these resu...Continue Reading

Citations

Dec 26, 2012·Antioxidants & Redox Signaling·Sabine LüthjeKerstin Wöltje
Apr 2, 2009·Journal of Experimental Botany·Suping ZhouTheodore W Thannhauser
Mar 6, 2009·Plant Physiology·Roberto De MicheleFiorella Lo Schiavo
Nov 16, 2010·Plant Physiology and Biochemistry : PPB·Belén Márquez-GarcíaFrancisco Córdoba
Jan 18, 2014·Plant, Cell & Environment·María Verónica Pérez-ChacaMaría C Romero-Puertas
Aug 30, 2014·Environmental Science and Pollution Research International·Antonella CastagnaRoberto Tognetti
Oct 25, 2016·Methods : a Companion to Methods in Enzymology·Cristina Ortega-VillasanteLuis E Hernández
Oct 13, 2009·Plant Signaling & Behavior·Suping ZhouTheodore W Thannhauser

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