Dehydroascorbic Acid Attenuates Ischemic Brain Edema and Neurotoxicity in Cerebral Ischemia: An in vivo Study

Experimental Neurobiology
Juhyun SongJong Eun Lee

Abstract

Ischemic stroke results in the diverse phathophysiologies including blood brain barrier (BBB) disruption, brain edema, neuronal cell death, and synaptic loss in brain. Vitamin C has known as the potent anti-oxidant having multiple functions in various organs, as well as in brain. Dehydroascorbic acid (DHA) as the oxidized form of ascorbic acid (AA) acts as a cellular protector against oxidative stress and easily enters into the brain compared to AA. To determine the role of DHA on edema formation, neuronal cell death, and synaptic dysfunction following cerebral ischemia, we investigated the infarct size of ischemic brain tissue and measured the expression of aquaporin 1 (AQP-1) as the water channel protein. We also examined the expression of claudin 5 for confirming the BBB breakdown, and the expression of bcl 2 associated X protein (Bax), caspase-3, inducible nitric oxide synthase (iNOS) for checking the effect of DHA on the neurotoxicity. Finally, we examined postsynaptic density protein-95 (PSD-95) expression to confirm the effect of DHA on synaptic dysfunction following ischemic stroke. Based on our findings, we propose that DHA might alleviate the pathogenesis of ischemic brain injury by attenuating edema, neuronal loss, a...Continue Reading

References

Jan 1, 1986·Annual Review of Nutrition·S Englard, S Seifter
Jan 1, 1985·British Journal of Anaesthesia·I Klatzo
Nov 1, 1981·Stroke; a Journal of Cerebral Circulation·J AstrupL Symon
Dec 1, 1993·The Journal of Cell Biology·M FuruseS Tsukita
Dec 1, 1996·Journal of Cardiovascular Risk·A R NessK T Khaw
May 2, 1997·The Journal of Biological Chemistry·M TakeuchiY Takai
Feb 12, 1998·The Journal of Clinical Investigation·D B AgusD W Golde
Jan 14, 1998·Neuron·E B Ziff
Jul 4, 1998·Brain Research. Brain Research Reviews·M B Kennedy
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·K MoritaS Tsukita
May 25, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Carr, B Frei
Apr 27, 2000·Trends in Neurosciences·M E Rice
Jun 22, 2000·Nature Neuroscience·H HusiS G Grant
Jul 6, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·F PuskasA Perl
Jul 25, 2000·Journal of Chromatography. a·J C Deutsch
May 24, 2001·Progress in Neuro-psychopharmacology & Biological Psychiatry·S P MahadikH Lal
Oct 13, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·D Attwell, S B Laughlin
Jan 31, 2002·Annual Review of Medicine·C Stapf, J P Mohr
Jul 4, 2002·The Journal of Physiology·Peter AgreSøren Nielsen
Sep 11, 2002·FEBS Letters·John X Wilson
Sep 11, 2003·Journal of Neurotrauma·Angela MuellnerGerhard F Hamann
Dec 13, 2003·Stroke; a Journal of Cerebral Circulation·Concepción Sánchez-MorenoAntonio Martin
Jan 26, 2005·Journal of Neuroscience Research·Eun Joo KimBae Hwan Lee
Dec 24, 2005·Nature Reviews. Neuroscience·N Joan AbbottElisabeth Hansson
Jan 16, 2007·The FEBS Journal·Carole L Linster, Emile Van Schaftingen
Feb 20, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Olga C ColganPhilip M Cummins
Mar 6, 2007·Progress in Retinal and Eye Research·Noboru Toda, Megumi Nakanishi-Toda
Jun 2, 2007·Amino Acids·I SaviniM V Catani
Sep 21, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jing ZhaoPramod K Dash
Nov 27, 2007·Biochemical and Biophysical Research Communications·Eun Jin KimBae Hwan Lee
Dec 22, 2007·Ultrastructural Pathology·Francesca PapadopulosGianandrea Pasquinelli
Jun 12, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Chikako NitoPak H Chan
Dec 20, 2008·Handbook of Experimental Pharmacology·Zsolt ZadorGeoffrey T Manley

❮ Previous
Next ❯

Citations

Mar 13, 2016·Medical Hypotheses·Reynold Spector
Apr 11, 2017·Expert Opinion on Pharmacotherapy·Brandon R ChristopheE Sander Connolly
Jun 28, 2017·Nutrients·Fiammetta MonacelliAlessio Nencioni
Mar 22, 2018·Critical Care : the Official Journal of the Critical Care Forum·Angelique M E Spoelstra-de ManHeleen M Oudemans-van Straaten
May 5, 2017·Journal of Central Nervous System Disease·Hrvoje BrzicaPatrick T Ronaldson
Feb 25, 2020·International Journal of Vascular Medicine·Kelston Paulo Felice de SalesFlávio Nigri
May 21, 2019·Free Radical Research·Pietro ScicchitanoMarco Matteo Ciccone
Feb 6, 2021·Journal of Neurotrauma·Lauren KangisserMark P Plummer
Aug 8, 2021·International Journal of Molecular Sciences·Natalia CichonMichal Bijak

❮ Previous
Next ❯

Methods Mentioned

BETA
dissecting

Software Mentioned

Quantity One
Image J
SPSS

Related Concepts

Related Feeds

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Blood Brain Barrier Regulation in Health & Disease

The blood brain barrier is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the blood brain barrier and changes in permeability allow pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research in this field.

Acute Stroke

A stroke occurs when blood supply to the brain is interrupted depriving the brain of oxygen and nutrients. This feed focuses cerebrovascular accidents including ischemic and paralytic stroke.

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.