Dehydroepiandrosterone and its metabolites: differential effects on androgen receptor trafficking and transcriptional activity

The Journal of Steroid Biochemistry and Molecular Biology
Qianxing MoNeal G Simon

Abstract

Dehydroepiandrosterone (DHEA) is a multi-functional steroid that has been implicated in a broad range of biological effects in humans and rodents. Recent studies demonstrated that DHEA acts genomically through the androgen receptor (AR) in addition to its well-known effects on cell surface receptors. However, the relative contribution of DHEA and its major metabolites, including DHEA-Sulfate (DHEA-S), 7alpha-OH-DHEA, 7beta-OH-DHEA, 7-oxo-DHEA, androstenedione (Adione), and androstenediol (Adiol), in the production of genomic effects remains controversial, in part because the metabolism of DHEA varies in different cells and tissues. In the current study, the ability of DHEA and its metabolites to promote AR intracellular trafficking and regulate AR-mediated reporter gene expression, which are characteristic effects of androgens, was determined. Intracellular trafficking of AR-GFP protein was assessed in COS-7 cells while AR transcriptional activity was tested in CV-1 cells transiently co-transfected with AR expression plasmid and an MMTV-ARE-CAT reporter. The results demonstrated that DHEA, the 3beta-HSD metabolite Adione, and the 17beta-HSD metabolite Adiol, were androgenic. Each promoted AR-GFP intracellular trafficking, the f...Continue Reading

References

Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·V G KimonidesJ Herbert
Sep 30, 1999·The New England Journal of Medicine·W ArltB Allolio
Feb 9, 2000·Frontiers in Neuroendocrinology·N A Compagnone, S H Mellon
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·E E BaulieuF Forette
Nov 5, 2002·The Journal of Clinical Endocrinology and Metabolism·Catherine M GordonMeryl S LeBoff
Apr 12, 2003·Neurology·O M WolkowitzUNKNOWN DHEA-Alzheimer's Disease Collaborative Research
Jun 5, 2003·Molecular and Cellular Endocrinology·Hong CuiDenise D Belsham
Jun 6, 2003·Best Practice & Research. Clinical Endocrinology & Metabolism·O T Wolf
Oct 9, 2003·Mayo Clinic Proceedings·Ketan K Dhatariya, K Sreekumaran Nair
Oct 11, 2003·World Journal of Urology·Jacques Buvat
Oct 17, 2003·Drugs & Aging·Sylvie Legrain, Laurence Girard
Jul 14, 2004·Brain Research. Molecular Brain Research·Qianxing MoNeal G Simon
Jul 23, 2004·Aging Cell·Wiebke Arlt, Martin Hewison
Nov 13, 2004·Hormone Research·Hajime NawataTetsuhiro Watanabe
Jan 7, 2005·Seminars in Reproductive Medicine·Fernand Labrie
Mar 8, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Peter H JellinckKaren Bulloch
Mar 24, 2005·Treatments in Endocrinology·Deborah R Cameron, Glenn D Braunstein
Apr 19, 2005·Treatments in Endocrinology·Dennis T Villareal

❮ Previous
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Citations

Oct 24, 2009·Langenbeck's Archives of Surgery·Yves HarderMichael D Menger
Aug 30, 2012·The Journal of Pharmacology and Experimental Therapeutics·Marco Brotto, Eduardo L Abreu
May 3, 2013·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Mohsen VosooghiAlireza Foroumadi
Apr 10, 2014·Eating and Weight Disorders : EWD·Senay TopsakalHilmi Dogu
Dec 17, 2015·Acta Physiologica·Pontus B Persson, Anna Zakrisson
Sep 29, 2011·Neuroscience·M PesaresiR C Melcangi
Feb 26, 2011·Steroids·Matheus Parmegiani JahnLuiz Carlos Kucharski
Dec 28, 2010·Molecular and Biochemical Parasitology·Shreedhara GuptaPaul A M Michels
Mar 17, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Matheus Parmegiani JahnLuiz Carlos Kucharski
May 12, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Qianxing MoNeal G Simon
Jul 1, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Nicole A DumasCharles N Falany
Jun 3, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Nicole H UrbanMatthew J Beckman
Jun 13, 2015·Journal of Biomolecular Structure & Dynamics·Jing-Rong FanHong-Xing Zhang
Mar 24, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Silvia GiattiRoberto Cosimo Melcangi
May 6, 2015·Nature Reviews. Endocrinology·Aya Shohat-TalNorbert Gleicher
Apr 10, 2012·Biochimica Et Biophysica Acta·Johan Lundqvist, Maria Norlin
Jan 18, 2018·Journal of Cellular Physiology·Hong-Li XieJing-He Tan
Nov 20, 2009·Cell Biochemistry and Function·Maria Helena Vianna Metello JacobMaria Flavia Marques Ribeiro
Nov 13, 2008·The Journal of Clinical Endocrinology and Metabolism·Thomas RemerStefan A Wudy
Jun 21, 2018·Frontiers in Endocrinology·Daniel J TobianskyKiran K Soma
Jul 1, 2017·Journal of Neurochemistry·M Zeeshan QaiserJonathan P Fry
Apr 15, 2020·Animals : an Open Access Journal From MDPI·Katiuska SatuéPietro Medica
May 11, 2017·Journal of Neuroendocrinology·T-V Nguyen
Jan 18, 2020·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Marike GabrielsonPer Hall
Feb 27, 2017·Cancer Research·Jeffrey D ThomasFelix J Kim
Sep 21, 2019·Biochemical and Biophysical Research Communications·Karl W SmithDiego F Cobice
Jun 29, 2017·Clinical Genitourinary Cancer·Terence W FriedlanderCharles J Ryan

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