PMID: 9449250Aug 1, 1997Paper

Dehydroepiandrosterone stimulates the estrogen response element

The Journal of Steroid Biochemistry and Molecular Biology
J M BruderM Oettel

Abstract

Studies suggest that the steroid, dehydroepiandrosterone (DHEA) can exert effects directly, in addition to its indirect role serving as a precursor for other steroids such as androgens and estrogens. Because DHEA is one of the most abundant adrenal steroids secreted in man, we investigated the functional activity of DHEA on the classic estrogen response element (ERE) in the presence of the estrogen receptor (ER) in transiently transfected cells. GT1-7 hypothalamic neuronal cells, devoid of the estrogen receptor, were transiently transfected with the estrogen receptor expression plasmid (HEGO) and the estrogen response element luciferase (ERELUC) reporter vector. As expected, a dose-response stimulation of luciferase activity was observed in cells treated with estradiol. Concentrations of estradiol from 10(-10)-10(-6) M resulted in a 136-195 percent increase in luciferase activity compared with control. A dose-response stimulation was also observed in the cells treated with DHEA. A maximum stimulation of 177 percent increase in luciferase activity compared with control was observed with DHEA at a concentration of 10(-5) M. Both the estradiol and DHEA stimulation of ERE luciferase activity was inhibited by the estrogen receptor a...Continue Reading

References

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Citations

Aug 6, 2002·Molecular and Cellular Endocrinology·Marcello MaggioliniSebastiano Andò
Jun 5, 2003·Molecular and Cellular Endocrinology·Hong CuiDenise D Belsham
Apr 9, 2002·Journal of Toxicology and Environmental Health. Part a·Seung Jun KwackKui Lea Park
Dec 17, 2014·Fiziologiia cheloveka·N P Goncharov, G V Katsia
Apr 25, 2009·Molecular Endocrinology·Linda L GrasfederDonald P McDonnell
Apr 19, 2005·Treatments in Endocrinology·Dennis T Villareal
May 6, 2014·The Journal of Steroid Biochemistry and Molecular Biology·Marcello MaggioGian Paolo Ceda
Nov 6, 2012·Steroids·Kristy K Michael MillerRussell A Prough
Feb 26, 2016·Journal of Molecular Endocrinology·Russell A ProughCarolyn M Klinge
Nov 13, 2004·American Journal of Physiology. Endocrinology and Metabolism·Julia T ArnoldMarc R Blackman
Jun 18, 1999·The Journal of Clinical Endocrinology and Metabolism·W ArltB Allolio
Mar 17, 2000·Proceedings of the Society for Experimental Biology and Medicine·K P NephewS A Khan

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