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Dehydroepiandrosterone sulfate in plasma: hydrolysis, extraction and radioimmunoassay

Steroids

Sep 1, 1976

Michael H Metcalf

PMID: 136071

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Abstract

At pH 4.5, the hydrolysis of 3beta-hydroxy-5-androsten-17-one 3-sulfate (DHA-SO4) to DHA was complete within 75 min at 120 degrees or 4h at 100 degrees. In the same conditions, the 3alpha-SO4 of androsterone was stable, and only 8.5% of the 3beta-SO4 of epiandrosterone hydrolysed to epi...read more

Mentioned in this Paper

Epiandrosterone
Dehydroepiandrosterone Sulfate
Androsterone Assay
Dehydroepiandrosterone Sulfate Measurement (Procedure)
Hydrolysis
Epiandrosterone Assay
Epicholesterol
Cholesterol Measurement Test
Glycol
Androstenes
Paper Details
References
  • References4
  • Citations21
123

Dehydroepiandrosterone sulfate in plasma: hydrolysis, extraction and radioimmunoassay

Steroids

Sep 1, 1976

Michael H Metcalf

PMID: 136071

DOI:

Abstract

At pH 4.5, the hydrolysis of 3beta-hydroxy-5-androsten-17-one 3-sulfate (DHA-SO4) to DHA was complete within 75 min at 120 degrees or 4h at 100 degrees. In the same conditions, the 3alpha-SO4 of androsterone was stable, and only 8.5% of the 3beta-SO4 of epiandrosterone hydrolysed to epi...read more

Mentioned in this Paper

Epiandrosterone
Dehydroepiandrosterone Sulfate
Androsterone Assay
Dehydroepiandrosterone Sulfate Measurement (Procedure)
Hydrolysis

Related Papers

Paper Details
References
  • References4
  • Citations21
123

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