Dehydroepiandrosterone-sulfate inhibits nuclear factor-kappaB-dependent transcription in hepatocytes, possibly through antioxidant effect

The Journal of Clinical Endocrinology and Metabolism
Yasumasa IwasakiNobuo Nakashima

Abstract

Dehydroepiandrosterone (DHEA) and DHEA-sulfate (DHEAS), the representative sex steroid precursors, are postulated to have antiinflammatory effects, although the molecular background remains unknown. In this study, we examined the effects of these sex steroid precursors on cytokine-induced, nuclear factor-kappaB (NF-kappaB)-mediated transcription. The HuH7 human hepatocyte cell line was stably transfected with an NF-kappaB-luciferase reporter gene or transiently transfected with other representative response elements-luciferase fusion genes, and the effects of DHEA/DHEAS on proinflammatory cytokine-induced transcription were estimated by luciferase assay. The results showed that DHEA/DHEAS potently inhibited TNF-alpha-induced NF-kappaB-dependent transcription in a time- and dose-dependent manner. The effect was more obvious for DHEAS than for DHEA, and both steroids preferentially inhibited the cytokine-stimulated rather than basal NF-kappaB-mediated transcription. Similar effects were observed in activator protein-1-dependent but not constitutive Rous sarcoma virus promoter-dependent transcription. Two major downstream products of the sex steroid precursors, estradiol and testosterone, had no effect, indicating that the observe...Continue Reading

Citations

May 13, 2009·The Journal of Biological Chemistry·Min Lian, Xiaofeng Zheng
May 18, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jonathan D KurtisLuz P Acosta
Jun 27, 2006·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Marcello MaggioLuigi Ferrucci
Sep 9, 2006·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Anne R CappolaLinda P Fried
Aug 12, 2008·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Anne R CappolaLuigi Ferrucci
May 13, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Noriyuki HommaMasahiko Oka
Sep 2, 2008·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Pei LuoZheng-Ji Mo
May 30, 2013·Indian Journal of Human Genetics·S I DolomatovA Skaliy
Apr 5, 2008·Der Internist·D Heutling, H Lehnert
Jul 9, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Elen A ChavesMarcus F Oliveira
Jul 24, 2014·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Julia StarkGabor Bekesi
Mar 8, 2012·Toxicology Mechanisms and Methods·Vaithinathan SelvarajuGautam Maulik
Nov 4, 2011·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Yavuz AlbayrakBunyami Unal
May 28, 2015·Theoretical Biology & Medical Modelling·Stephanie SeneffGlyn Wainwright
Sep 29, 2007·Endocrinology·Manuela AragnoGiuseppe Boccuzzi
Apr 16, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Abdallah AlzoubiMasahiko Oka
Mar 19, 2020·Current Alzheimer Research·Dubravka S StracNela Pivac
May 24, 2011·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Chunshu JiaLiqun Ren

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.