Abstract
Beneficial effects of dehydroepiandrosterone (DHEA) supplement on age-associated chronic diseases such as cancer, cardiovascular disease, insulin resistance and diabetes, have been reported. However, its mechanism of action in hepatocellular carcinoma in vivo has not been investigated in detail. We have previously shown that during hepatocellular carcinogenesis, DHEA treatment decreases formation of preneoplastic glutathione S-transferase placental form-positive foci in the liver and has antioxidant effects. Here we aimed to determine the mechanism of actions of DHEA, in comparison to vitamin E, in a chemically-induced hepatocellular carcinoma model in rats. Sprague-Dawley rats were administered with control diet without a carcinogen, diets with 1.5% vitamin E, 0.5% DHEA and both of the compounds with a carcinogen for 6 weeks. The doses were previously reported to have anti-cancer effects in animals without known toxicities. With DHEA treatment, cytosolic malate dehydrogenase activities were significantly increased by ~5 fold and glucose 6-phosphate dehydrogenase activities were decreased by ~25% compared to carcinogen treated group. Activities of Se-glutathione peroxidase in the cytotol was decreased significantly with DHEA tr...Continue Reading
References
Jan 1, 1978·Methods in Enzymology·A L Tappel
Feb 1, 1990·Proceedings of the Society for Experimental Biology and Medicine·M MarreroL Milewich
Jun 1, 1990·Liver·W M FrederiksJ James
Jan 25, 1985·Science·P A Cerutti
Mar 1, 1988·Carcinogenesis·N ItoT Masui
Feb 1, 1969·American Journal of Ophthalmology·M S Nirankari, D Singh
Mar 4, 1969·Biochimica Et Biophysica Acta·O Stokke
Jan 7, 1998·Cell Biochemistry and Function·L LadriereW J Malaisse
May 8, 2001·Archives of Biochemistry and Biophysics·J L FitzpatrickR A Prough
Feb 19, 2004·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Andreas S Müller, Josef Pallauf
May 25, 2005·International Endodontic Journal·J W KimZ Cai
Jun 19, 2007·Lipids·Sumei ZhaoWeihua Chen
Jul 24, 2007·Nature Clinical Practice. Endocrinology & Metabolism·Fernand Labrie
Aug 11, 2007·Annales d'endocrinologie·B AllolioS Hahner
Oct 4, 2007·American Journal of Physiology. Endocrinology and Metabolism·Claude RobergeNicole Gallo-Payet