PMID: 11311737Apr 20, 2001Paper

Delay of neutrophil apoptosis by the neuropeptide substance P: involvement of caspase cascade

Peptides
S BöckmannL Jonas

Abstract

Neutrophil apoptosis is an important event in the resolution of inflammation. The role of substance P (SP) in neutrophil apoptosis has not been previously investigated. We found that substance P delays apoptosis in neutrophils. Human neutrophils were isolated and cultured up to 24 hours. Apoptosis was detected by light and electron microscopy, as well as DNA-fragmentation assays. Substance P delayed the spontaneous apoptosis of neutrophils at 6, 12, 18 and 24 hours in a dose-dependent fashion in the range of 10-100 microM. Whereas the both peptide neurokinin-1 (NK-1) receptor antagonists [D-Pro(2), D-Trp(7,9)]-SP and GR 82334 inhibited the substance P effect on neutrophils, the nonpeptide NK(1) receptor antagonist L-703.606 itself, an analogue of CP-96,345, induced apoptosis of neutrophils. Surprisingly, the effect of L-703.606 could be prevented by substance P. Western blotting results showed that the neuropeptide substance P inhibited the spontaneous apoptosis-associated caspase-3 activation in the same concentration range as described above. Parallel the inhibition of cleavage of focal adhesion kinase (FAK), a substrate of caspases could be observed by substance P. In conclusion, our results extend the range of biological ef...Continue Reading

References

Nov 1, 1992·American Journal of Respiratory Cell and Molecular Biology·J A Nadel
Jan 1, 1992·Annals of the New York Academy of Sciences·J A Nadel
Jan 1, 1992·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·P J Barnes
Sep 4, 1992·European Journal of Pharmacology·A W SchmidtJ Heym
Mar 1, 1995·American Journal of Respiratory and Critical Care Medicine·M TomakiK Shirato
Sep 1, 1995·General Pharmacology·C A Maggi
Nov 1, 1994·Immunology Today·D M McKay, J Bienenstock
Jul 1, 1993·The American Review of Respiratory Disease·A G DriverS J Mustafa
Jun 1, 1993·International Immunology·Y TakedaF Sendo
Jul 1, 1993·The Journal of Clinical Investigation·M K WhyteC Haslett
Mar 28, 1996·European Journal of Pharmacology·T TanabeC Inagaki
Nov 5, 1997·The Journal of Biological Chemistry·L P WenG D Rosen
Jun 27, 1998·The Journal of Biological Chemistry·F G GervaisS Roy
Feb 6, 1999·The Journal of Biological Chemistry·C WardA G Rossi

❮ Previous
Next ❯

Citations

Jun 27, 2014·ISRN Radiology·Ayse AralasmakAlpay Alkan
Feb 9, 2005·British Journal of Pharmacology·Julie KeebleSusan Diana Brain
Sep 27, 2003·Journal of Neurochemistry·F LallemendB Malgrange
Mar 27, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Mi-Hyun KangYoungsook Son
Aug 31, 2004·Journal of Cellular Physiology·Terence M O'ConnorFergus Shanahan
Mar 3, 2020·Journal of Cellular Biochemistry·Sahar KhorasaniSeyed Isaac Hashemy
Jul 29, 2010·Ocular Immunology and Inflammation·Linda D Hazlett, Robert L Hendricks
Jun 30, 2019·International Journal of Molecular Sciences·Tsung-Chuan HoYeou-Ping Tsao
Dec 8, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Carla M PradoIolanda F L C Tibério
May 13, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aneta Ostróżka-Cieślik, Barbara Dolińska
Nov 18, 2003·The Journal of Pharmacology and Experimental Therapeutics·Renate BangGisa Tiegs
Aug 31, 2019·Cancers·Miguel MuñozRafael Coveñas
Jun 15, 2017·Frontiers in Cellular and Infection Microbiology·Irina MiraldaKenneth R McLeish

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Caspases in Metabolic Diseases

Caspases, the family of cysteine proteases are involved in programmed cell death, but their role in metabolic diseases, inflammation and immunity has been of interested. Discover the latest research on caspases in metabolic diseases here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis