PMID: 7521132Aug 1, 1994Paper

Delayed activation of single mechanosensitive channels in Lymnaea neurons

The American Journal of Physiology
D L Small, C E Morris

Abstract

Some stretch-activated (SA) channels challenged with suction jumps exhibit adaptation, a dynamic behavior that can be overlooked because of its mechanical fragility. In previous studies of neuronal SA K channels, we detected no adaptation, but the protocols used were not designed to detect dynamics. Here, we reproduce the adaptation seen by others in Xenopus SA cationic (Cat) channels but show that, with the same protocol, no adaptation occurs with SA K channels. Instead, SA K channels exhibit a different dynamic behavior, delayed activation. Lymnaea SA K channels subjected to pressure jumps responded after a 1- to 4-s delay with a gradual, rather than abrupt, onset of activation. The delay was pressure dependent and was longer for patches from older cultured neurons. Delayed responses were fragile like SA Cat channel adaptation; they disappeared with repeated stimuli. Cytochalasin D decreased the delay and increased the stretch activation of SA K channels. Unlike SA Cat channel adaptation, which occurs only at hyperpolarized potentials, SA K channel delay was not voltage dependent. We note that once SA Cat and SA K channels are "stripped" of their fragile (cytoskeleton-dependent?) dynamics, however, their gating behaviors show...Continue Reading

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Citations

Jan 1, 1997·Annual Review of Physiology·O P Hamill, D W McBride
Aug 12, 2000·Journal of Neurophysiology·X SuG F Gebhart
Mar 29, 2001·Physiological Reviews·O P Hamill, B Martinac
Apr 6, 2005·Clinical & Experimental Ophthalmology·Freny B KalapesiMinas T Coroneo
Feb 24, 2006·Journal of Neuroscience Research·Lin PiaoSeog Bae Oh
Jan 27, 2007·The Journal of Physiology·Thomas M Suchyna, Frederick Sachs
Dec 20, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Changyan XiePingbo Huang
Jan 1, 1995·British Journal of Pharmacology·D L Small, C E Morris
Feb 10, 1999·The American Journal of Physiology·X WanC E Morris
Oct 6, 2006·Pflügers Archiv : European journal of physiology·O P Hamill
Jul 10, 2001·American Journal of Physiology. Cell Physiology·T Y NakamuraM Shigekawa
Aug 7, 2009·American Journal of Physiology. Cell Physiology·Jun A WangCatherine E Morris
May 30, 2003·American Journal of Physiology. Cell Physiology·Shlomo Ben-Tabou De-LeonItzhak Nussinovitch
Jun 25, 2005·EMBO Reports·Inger LauritzenAmanda Jane Patel
Feb 9, 2000·American Journal of Physiology. Heart and Circulatory Physiology·T ZengF Sachs
Apr 20, 2010·Nature Cell Biology·Wei Kevin ZhangPingbo Huang
Feb 14, 2015·PloS One·Catherine E MorrisBéla Joós
May 7, 2003·Progress in Biophysics and Molecular Biology·Weizhen Niu, Frederick Sachs
Nov 1, 1996·Glia·C L Bowman, J W Lohr
Jul 2, 1998·Biophysical Journal·J Akinlaja, F Sachs
Nov 5, 2002·Biophysical Journal·Barbara CalabreseCatherine E Morris
Dec 8, 2009·Trends in Neurosciences·Masha Prager-Khoutorsky, Charles W Bourque

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