Delayed activation of the mannose receptor following synthesis. Requirement for exit from the endoplasmic reticulum.

The Journal of Biological Chemistry
S E PontowP D Stahl

Abstract

The macrophage mannose receptor specifically recognizes proteins and particles bearing mannose terminal oligosaccharide chains. In the present study, we examined the ability of newly synthesized receptor to bind ligand. Human monocyte-derived macrophages were pulse-labeled with [35S]Met and prepared for affinity chromatography on mannose-Sepharose. Mannose receptor in the flow-through and eluted fractions was detected by fluorography following immunoprecipitation and gel electrophoresis. Labeled mannose receptor was found exclusively in the nonbinding fraction until 10 min of chase. Following a 60-min chase, 67-86% of newly synthesized receptor was precipitated from the bound column fraction. The half-time for development of receptor binding activity was determined to be 35-40 min compared with a 45-min half-time for development of endoglycosidase H resistance. Mannose receptor synthesized by cells incubated in brefeldin A required more than 120 min to acquire endoglycosidase H resistance and maximal binding activity. Inhibitors of N-linked oligosaccharide processing or of O-glycosylation had no effect on the development of mannose receptor binding activity. Monensin prevented terminal sialylation of oligosaccharide side chains...Continue Reading

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Citations

Apr 2, 1998·Current Opinion in Immunology·P D Stahl, R A Ezekowitz
May 9, 2000·Journal of Virology·P C ReadingE M Anders
Feb 13, 2008·Traffic·Markus LanghansFernando Aniento
Jul 14, 1998·Blood Reviews·G A GrabowskiR Wenstrup
Jun 29, 2005·The Journal of Biological Chemistry·Yunpeng SuLuisa Martinez-Pomares
Apr 17, 1999·The Journal of Biological Chemistry·C Alvarez-Dominguez, P D Stahl
Jul 18, 2000·The Journal of Biological Chemistry·A SchweizerJ Rohrer
Dec 16, 1998·The Journal of Biological Chemistry·C Alvarez-Dominguez, P D Stahl
Sep 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alison A BeharkaLarry S Schlesinger
Sep 1, 2006·Neurochemical Research·Giuseppe VerdileRalph N Martins

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