Delayed-relaxed response explained by hyperactivation of RelE

Molecular Microbiology
Susanne K Christensen, Kenn Gerdes

Abstract

Escherichia coli encodes two rel loci, both of which contribute to the control of synthesis of macromolecules during amino acid starvation. The product of relA (ppGpp synthetase I) is responsible for the synthesis of guanosine tetraphosphate, ppGpp, the signal molecule that exerts stringent control of stable RNA synthesis. The second rel locus, relBE, was identified by mutations in relB that confer a so-called 'delayed-relaxed response' characterized by continued RNA synthesis after a lag period of approximately 10 min after the onset of amino acid starvation. We show here that the delayed-relaxed response is a consequence of hyperactivation of RelE. As in wild-type cells, [ppGpp] increased sharply in relB101 relE cells after the onset of starvation, but returned rapidly to the prestarvation level. RelE is a global inhibitor of translation that is neutralized by RelB by direct protein-protein interaction. Lon protease activates RelE during amino acid starvation by degradation of RelB. We found that mutations in relB that conferred the delayed-relaxed phenotype destabilized RelB. Such mutations confer severe RelE-dependent inhibition of translation during amino acid starvation, indicating hyperactivation of RelE. Hyperactivation...Continue Reading

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Citations

May 3, 2005·Nature Reviews. Microbiology·Kenn GerdesAnders Løbner-Olesen
Mar 21, 2007·Applied and Environmental Microbiology·Barbara O GvakhariaDaniel J Arp
Jul 4, 2006·Journal of Bacteriology·Amy L SpoeringKim Lewis
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Jun 29, 2006·Molecular Microbiology·Ciarán Condon
Oct 6, 2006·Molecular Microbiology·Mikkel Christensen-Dalsgaard, Kenn Gerdes
Dec 1, 2009·Molecular Microbiology·Mikkel Christensen-DalsgaardKenn Gerdes
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