Delayed resolution of bleomycin-induced pulmonary fibrosis in absence of MMP13 (collagenase 3)

American Journal of Physiology. Lung Cellular and Molecular Physiology
Sandra CabreraAnnie Pardo

Abstract

Matrix metalloprotease 13 (MMP13) deficiency in pulmonary fibrosis has described contradictory phenotypes on inflammatory and fibrotic responses after lung injury, and its role during lung fibrosis resolution is still undefined. MMP13 has been considered the main collagenase in rodents, and the remodeling of fibrillar collagen is widely attributed to the action of this enzyme. In this study we aimed to explore the role of MMP13 during lung fibrosis progression and resolution. Lung fibrosis was induced by intratracheal instillation, and inflammatory, fibrotic, and resolution stages were evaluated in Mmp13-null and wild-type (WT) mice. Bronchoalveolar lavage fluid was taken for cytokine array analysis and activity of gelatinases. Our results showed that MMP13 is upregulated mainly during two stages after lung injury, inflammation and resolution of fibrosis, and it is mainly expressed by alveolar and interstitial macrophages. Mmp13-null mice exhibited more extensive inflammation at 7 days after bleomycin treatment, and it was characterized by increased macrophage infiltration and significant alterations in proinflammatory cytokines. We also documented that Mmp13-deficient mice experienced more severe and prolonged lung fibrosis co...Continue Reading

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Citations

Apr 23, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Wells B LaRiviereYimu Yang
Oct 8, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Adam GerberJeanine D'Armiento
Jan 12, 2021·Expert Review of Proteomics·Ting ZhangChandra Mohan
Dec 4, 2020·American Journal of Respiratory Cell and Molecular Biology·Xiaoyun LiYingze Zhang
Dec 15, 2019·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Sushweta MahalanobishParames C Sil
Apr 4, 2021·International Journal of Molecular Sciences·Luis PlacidoAnnie Pardo
Jun 12, 2021·Cell Death & Disease·Tingting ChenHaihai Liang

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Methods Mentioned

BETA
bronchoalveolar lavage
PCR
light microscopy
FRET
Protein Assay
antibody array
bronchoalveolar
lavage
zymography
electrophoresis

Software Mentioned

NIS
Graph
Quantity One
Elements
Prism
- Pad

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