Delayed testicular aging in pituitary adenylate cyclase-activating peptide (PACAP) null mice

Proceedings of the National Academy of Sciences of the United States of America
Arnaud LacombeEric Vilain

Abstract

Age-related decline in male sex hormones is a direct consequence of testicular aging. These changes in the hormonal complement cause physiological disturbances affecting the quality of life for millions of aging men. To assess the influence on testicular aging of pituitary adenylate cyclase-activating peptide (PACAP), a polypeptide that regulates testicular steroidogenesis in vitro, we compared the testicular structure and function between C57BL/6 wild-type and PACAP-/- male mice, at 4 and 15 months of age. We show that, in 4-month-old PACAP-/- mice, steroidogenesis (evaluated by levels of testosterone, steroidogenic acute regulatory protein, 3beta-hydroxysteroid dehydrogenase, and P450c17) was impaired. However, the testicular structure of these animals was not affected. At 15 months of age, wild-type testis displayed typical signs of aging (patchy seminiferous tubules, germ cell depletion, and vacuolization), whereas testicular structure was remarkably well conserved in PACAP-/- animals. The depletion of germ cells found in wild-type animals was associated with a higher content of peroxynitrites, a marker of reactive oxygen species, and a higher number of apoptotic cells compared with PACAP-/- mice. Our results show that test...Continue Reading

References

Jan 1, 1982·Journal of Reproduction and Fertility·R G GosdenD W Davidson
Oct 1, 1994·Endocrine Reviews·J M Saez
Dec 1, 1993·Endocrinology·C WangA P Sinha-Hikim
Feb 24, 1998·Biology of Reproduction·F El-GehaniI Huhtaniemi
Oct 10, 1998·The American Journal of Psychiatry·H Sternbach
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·H Chen, B R Zirkin
Jun 17, 2000·Biochimica Et Biophysica Acta·D M Stocco
Dec 13, 2000·Experimental Gerontology·J D Veldhuis
Feb 22, 2001·Annual Review of Physiology·D M Stocco
Nov 1, 2002·Regulatory Peptides·Norihito ShintaniAkemichi Baba
Jan 22, 2003·Journal of the American Geriatrics Society·David A Gruenewald, Alvin M Matsumoto
Oct 31, 2003·Proceedings of the National Academy of Sciences of the United States of America·Fu-Ping ZhangIlpo Huhtaniemi
Nov 25, 2003·The American Journal of Pathology·Reza J RasoulpourKim Boekelheide
Jan 16, 2004·Trends in Pharmacological Sciences·Axel Kamischke, Eberhard Nieschlag
Jun 26, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Asim KhanCho Y Pang
Jun 26, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·C S ColwellJ A Waschek
Jul 10, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Larry A SlomowitzKaren A Munger
Oct 12, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Laura C GeranAlan C Spector
Dec 2, 2004·American Journal of Physiology. Endocrinology and Metabolism·Johannes D VeldhuisAli Iranmanesh
Dec 21, 2004·Redox Report : Communications in Free Radical Research·Masayasu InoueKozo Utsumi
Jan 22, 2005·Annual Review of Medicine·Rabih A Hijazi, Glenn R Cunningham

❮ Previous
Next ❯

Citations

Jun 8, 2010·Journal of Molecular Neuroscience : MN·David AlexandreJames A Waschek
May 29, 2012·Journal of Molecular Neuroscience : MN·R BrubelD Reglodi
Jul 12, 2007·The Journal of Biological Chemistry·Maria AlmeidaStavros C Manolagas
Dec 26, 2008·Human Reproduction Update·Charles Gyamera-Acheampong, Majambu Mbikay
Dec 31, 2010·Biology of Reproduction·Stephen J Winters, Joseph P Moore
May 9, 2012·Seminars in Immunology·Ivan K ChinnGregory D Sempowski
Oct 12, 2015·Journal of Molecular Neuroscience : MN·Andrea TamasZalan Szanto
Nov 5, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·D ReglodiT Juhasz
Dec 21, 2018·Andrologia·María Susana Theas
Oct 10, 2006·Médecine sciences : M/S·Arnaud LacombeEric Vilain
Nov 4, 2016·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Serra UcerStavros C Manolagas
Aug 15, 2018·Journal of Molecular Neuroscience : MN·Catalina Abad, Yossan-Var Tan
Aug 14, 2020·International Journal of Molecular Sciences·Vince SzegeczkiTamás Juhász
Aug 9, 2020·Frontiers in Endocrinology·Denes TothDora Reglodi
Oct 23, 2018·GeroScience·D ReglodiA Bardosi
Jun 21, 2020·Molecular and Cellular Endocrinology·Stephen J Winters, Joseph P Moore

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis