Delayed-type hypersensitivity-like mechanisms dominate late acute rejection episodes in renal allograft recipients

Transplantation
S Ode-HakimP Reinke

Abstract

Episodes of acute rejection (aRx) may occur in functional renal allografts even at a very late stage post-Tx. Histopathology in early and late aRx looks quite similar--however, there is a slower deterioration of graft function in late aRx, suggesting that pathogenetic immune mechanisms are different. In order to investigate this phenomenon we studied the gene expression pattern (IL-1beta, 2, 4, 8, 10, IFNgamma, TNFalpha, GrnA, IL-2R p55/p75) in PBMC and core biopsies from long-term renal allograft recipients with histologically proven late aRx and compared it with transplant and non-transplant controls using a semiquantitative RT-PCR technique. PBMC and graft-infiltrating cells of patients with late aRx showed an upregulation, especially of IFN-gamma, IL-4, IL-10, and TNFalpha transcripts. While IL-2 mRNA was only detected in PBMC of two patients with late aRx who were not on cyclosporine, upregulation of intragraft IL-2 mRNA allowed the best discrimination between aRx and the other groups (sensitivity: 83%, specificity: 93%). In contrast to several reports on early Rx we did not notice an elevation of granzyme A transcripts in comparison with the controls, suggesting that cell-mediated inflammatory processes (CD4+ T cell-media...Continue Reading

References

Mar 1, 1992·Transplantation·M J DallmanP J Morris
Aug 1, 1992·Transplantation·C J WuC Clayberger
Feb 1, 1992·Transplantation·O M MartinezN L Ascher
Mar 1, 1991·Transplantation·C VandenbroeckeM G Tovey
Oct 1, 1991·Transplantation·T SablinskiJ W Kupiec-Weglinski
Oct 1, 1990·Transplantation·J S Pober, R S Cotran
Aug 1, 1989·Transplantation·H M Georgiou, D Bellgrau
Jul 1, 1984·Transplantation·P Häyry, E von Willebrand
Feb 1, 1995·European Journal of Immunology·M SchulzK Bürki
Apr 7, 1995·Cell·G Berke

❮ Previous
Next ❯

Citations

Apr 20, 2010·World Journal of Urology·Jens BedkeHermann-Josef Gröne
Dec 1, 1999·Transplantation Proceedings·N IshidoN Tanaka
Sep 10, 2002·Transplant Immunology·Benjamin A Bradley
Dec 9, 2000·Transplant International : Official Journal of the European Society for Organ Transplantation·A Reutzel-SelkeH D Volk
Jun 29, 2001·Transplant Infectious Disease : an Official Journal of the Transplantation Society·P ReinkeH D Volk
Mar 3, 2001·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·S SegererC E Alpers
Oct 21, 2015·Kidney Research and Clinical Practice·Myung-Gyu Kim
May 30, 2003·Pharmacological Reviews·K AsadullahH D Volk

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.