PMID: 12758168May 22, 2003Paper

Deleterious effect of peptide insertions in a permissive site of the AAV2 capsid

Virology
Anne M DouarOlivier Danos

Abstract

A permissive site for insertion of heterologous peptide sequences has been identified in the capsid proteins of AAV2. While attempting to use this site for insertion of a nuclear localization sequence, we have observed a drastic reduction in the yield of DNA-containing particles. ELISA analysis showed that capsid assembly was modestly affected, whereas genome encapsidation was more profoundly altered, a phenomenon we did not observed when a RGD peptide was inserted at the same location. Furthermore, the NLS viruses displayed poor transduction efficiency on HeLa and 293 cells. Altogether, these results indicate that the nature of the peptide inserted at position 587 in the capsid may have important consequences on both particle formation and infectivity.

References

Jun 7, 2000·The Journal of Clinical Investigation·D DuanJ F Engelhardt
Mar 23, 2001·The Journal of Biological Chemistry·R W WaltersJ Zabner
Jun 16, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·M GrifmanM D Weitzman
Jul 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Qing XieMichael S Chapman

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Citations

Oct 3, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kim Van VlietRichard O Snyder
Dec 15, 2015·Molecular Therapy. Methods & Clinical Development·Joseph M KelichWeidong Yang
Sep 7, 2006·The Journal of Gene Medicine·Daniel A WaterkampJürgen A Kleinschmidt
Aug 3, 2016·Angewandte Chemie·Rachel E KelemenAbhishek Chatterjee

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