PMID: 6411929Aug 5, 1983Paper

Deletion analysis of a complex promoter for a developmentally regulated gene from Bacillus subtilis

Journal of Molecular Biology
C D BannerR Losick

Abstract

SpoVG is a developmentally regulated gene from the spore-forming bacterium Bacillus subtilis. The transcription initiation region for spoVG consists of two overlapping promoters whose startpoints of RNA synthesis are ten base pairs apart (Moran et al., 1981a). These startpoints are separately utilized by two forms of RNA polymerase holoenzyme containing different species of B. subtilis sigma factor. We have constructed a series of deletion mutations that extend into the spoVG promoter region from the downstream and from the upstream directions. Transcription studies with these mutated promoters showed that the functional boundaries of the spoVG promoters extended from the region of the transcription startpoints into an upstream A + T-rich box, which was located 76 to 51 base pairs preceding the downstream startsite. We have unexpectedly discovered that propagation of the spoVG promoter region on a high copy number plasmid in B. subtilis interferes with the process of sporulation by impairing development at an early stage. This was not a general effect of promoter amplification, since the propagation on plasmids of two other strong Bacillus promoters had little or no effect on spore formation. Deletion analysis established that ...Continue Reading

References

Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S N CohenL Hsu
Sep 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·P SchaefferJ P Aubert
Sep 1, 1981·Cell·R Losick, J Pero

❮ Previous
Next ❯

Citations

Dec 5, 1985·Journal of Molecular Biology·U PeschkeS Le Grice
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J B RobertsonP Zuber
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·K FredrickJ D Helmann
Jan 1, 1984·Biotechnology & Genetic Engineering Reviews·R H Doi
Feb 20, 2004·Nucleic Acids Research·Wilfried J J Meijer, Margarita Salas
May 19, 2009·Journal of Bacteriology·Wan-Ju KeShih-Tung Liu
Dec 26, 2006·Journal of Bacteriology·Maria HadjifrangiskouTheresa M Koehler
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·S T EstremR L Gourse
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·S E AiyarW Ross
Jan 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Wilma Ross, Richard L Gourse
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·K Fredrick, J D Helmann
Aug 1, 1986·Molecular & General Genetics : MGG·S F Le GriceA L Sonenshein
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D W CowingC A Gross
Dec 24, 2013·Biochemical and Biophysical Research Communications·Xingliang PanZhiyang Dong
Jan 3, 1985·Nature·J WestphelingR Losick
Mar 1, 1993·Molecular Microbiology·M A MarahielP Zuber
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·R L GourseW Ross
Jan 1, 1986·Journal of Basic Microbiology·G Eitner-Mönke, R Manteuffel
May 29, 1999·Journal of Bacteriology·K Matsuno, A L Sonenshein
Jul 1, 1989·Journal of Bacteriology·M J Weickert, G H Chambliss
Oct 1, 1989·Journal of Bacteriology·M AlbanoD A Dubnau

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.