PMID: 9557722Apr 29, 1998Paper

Deletion analysis of a defective interfering Semliki Forest virus RNA genome defines a region in the nsP2 sequence that is required for efficient packaging of the genome into virus particles

Journal of Virology
C L WhiteN J Dimmock

Abstract

The 1,244-nucleotide genome of Semliki Forest virus (SFV) defective interfering (DI) RNA 19 (DI-19) is coterminal with the infectious genome and contains two major deletions. One deletion removes the end of the nsP1 gene and the beginning of the nsP2 gene, and the other removes the end of the nsP2 gene, the nsP3 and nsP4 genes, and all of the structural protein genes (M. Thomson and N. J. Dimmock, Virology 199:354-365, 1994). Like all DI SFV RNAs, DI-19 contains three regions that are conserved. Region a comprises the 5' terminus continuous with part of the nsP1 gene, region b comprises a central part of the nsP2 gene, and region c comprises the 3' terminus and the associated untranslated region. A deletion analysis of the 265-nucleotide b region (nucleotides 679 to 943, inclusive) was undertaken to determine its role in genome replication and packaging into DI virus particles. Deleted plasmids were constructed and transcribed, and the resulting DI RNAs were transfected into SFV-infected BHK cells. Putative progeny DI virus particles that had been released into the tissue culture fluid were then serially passaged in new monolayers together with added high-multiplicity SFV, and cells and tissue culture fluids were tested for the...Continue Reading

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Citations

Sep 29, 1999·The Journal of General Virology·G J AtkinsP Liljeström
Apr 20, 2001·Journal of Virology·U Skoging-Nyberg, P Liljeström
Jul 24, 2020·International Journal of Molecular Sciences·Kenneth Lundstrom
Mar 9, 2000·Journal of Virology·R MolenkampE J Snijder
Oct 15, 2003·Journal of Virology·Matthew J BickMargaret R MacDonald
Sep 19, 2020·Nature Communications·Rebecca S BrownMargaret Kielian
Mar 9, 2000·Journal of Virology·A A KhromykhE G Westaway
Oct 16, 2016·Journal of Neurovirology·Fan JiaFuqiang Xu
Feb 23, 2018·Viruses·Rebecca S BrownMargaret Kielian
Mar 21, 2018·Viruses·Adriano Mendes, Richard J Kuhn
May 15, 2019·Annual Review of Virology·Emmanuelle Genoyer, Carolina B López

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