Deletion analysis of Qbeta replicase. Participation of the carboxyl-terminal region of the beta-subunit protein in template recognition.

The Journal of Biological Chemistry
Yoshio Inokuchi, M Kajitani

Abstract

We have analyzed one of the functional domains of Qbeta replicase, an RNA-dependent RNA polymerase of RNA coliphage Qbeta. Deletion mapping analysis of the carboxyl-terminal region of the beta-subunit protein revealed that the terminal 18 amino acid residues (positions 571-588) are dispensable for the replicase reaction. Subsequent deletions up to the Ala-565 residue reduced the RNA polymerizing activity of the replicase in vivo but increased it in vitro. The mutant replicases with enhanced in vitro RNA polymerizing activity were found to have relaxed template specificity for ribosomal RNAs and cellular RNAs as well as Qbeta RNA. Deletions beyond the Ile-564 residue abolished both the RNA polymerizing activity and the binding ability to midivariant (MDV)-poly(+) RNA, a derivative of a natural template for Qbeta replicase, MDV-1 RNA. These results suggest that the carboxyl-terminal part of the beta-subunit participates in RNA recognition of Qbeta replicase.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1979·Annual Review of Biochemistry·T Blumenthal, G G Carmichael
Mar 11, 1991·Nucleic Acids Research·M Kajitani, A Ishihama
Jul 1, 1990·Journal of Biochemistry·Y Inokuchi, A Hirashima
Nov 11, 1988·Nucleic Acids Research·P Argos
Feb 20, 1989·Journal of Molecular Biology·D R MillsB D Binderow
Dec 1, 1987·Journal of Virology·Y Inokuchi, A Hirashima
Jul 11, 1988·Nucleic Acids Research·Y InokuchiA Hirashima
Apr 1, 1986·Journal of Biochemistry·Y InokuchiA Hirashima
Mar 15, 1973·Journal of Molecular Biology·L A Ball, P Kaesberg
Aug 10, 1968·Nature·M T Franze de FernandezJ T August
Jan 21, 1972·European Journal of Biochemistry·M Kondo, C Weissmann
Mar 1, 1983·Journal of Biochemistry·T NishiharaF R Kramer
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·D Brown, L Gold

❮ Previous
Next ❯

Citations

Oct 4, 2013·Nature Communications·Norikazu IchihashiTetsuya Yomo
Nov 19, 2015·Nucleic Acids Research·Heidi GytzCharlotte R Knudsen
Apr 7, 2007·The Journal of Biological Chemistry·Kazufumi HosodaTetsuya Yomo

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.