Oct 23, 1997

Deletion analysis of the fis promoter region in Escherichia coli: antagonistic effects of integration host factor and Fis

Journal of Bacteriology
Timothy S PrattRobert Osuna


Fis is a small DNA-binding and -bending protein in Escherichia coli that is involved in several different biological processes, including stimulation of specialized DNA recombination events and regulation of gene expression. fis protein and mRNA levels rapidly increase during early logarithmic growth phase in response to a nutritional upshift but become virtually undetectable during late logarithmic and stationary phases. We present evidence that the growth phase-dependent fis expression pattern is not determined by changes in mRNA stability, arguing in favor of regulation at the level of transcription. DNA deletion analysis of the fis promoter (fis P) region indicated that DNA sequences from -166 to -81, -36 to -26, and +107 to +366 relative to the transcription start site are required for maximum expression. A DNA sequence resembling the integration host factor (IHF) binding site centered approximately at -114 showed DNase I cleavage protection by IHF. In ihf cells, maximum cellular levels of fis mRNA were decreased more than 3-fold and transcription from fis P on a plasmid was decreased about 3.8-fold compared to those in cells expressing wild-type IHF. In addition, a mutation in the ihf binding site resulted in a 76 and 61%...Continue Reading

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Mentioned in this Paper

Gene Expression Regulation, Bacterial
Deoxyribonuclease I
Bacterial Proteins
Alkalescens-Dispar Group
Decompression Sickness
Transcription Initiation Site
Recombination, Genetic
Gene Deletion Abnormality
Cytokinesis of the Fertilized Ovum
Bending - Changing Basic Body Position

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