PMID: 8955386Dec 1, 1996Paper

Deletion analysis of the FliM flagellar switch protein of Salmonella typhimurium

Journal of Bacteriology
A S TokerR M Macnab

Abstract

The flagellar switch of Salmonella typhimurium and Escherichia coli is composed of three proteins, FliG, FliM, and FliN. The switch complex modulates the direction of flagellar motor rotation in response to information about the environment received through the chemotaxis signal transduction pathway. In particular, chemotaxis protein CheY is believed to bind to switch protein FliM, inducing clockwise filament rotation and tumbling. To investigate the function of FliM and its interactions with FliG and FliN, we engineered a series of 34 FliM deletion mutant proteins, each lacking a different 10-amino-acid segment. We have determined the phenotype associated with each mutant protein, the ability of each mutant protein to interfere with the motility of wild-type cells, and the effect of additional FliG and FliN on the function of selected FliM mutant proteins. Overall, deletions at the N terminus produced a counterclockwise switch bias, deletions in the central region of the protein produced poorly motile or nonflagellate cells, and deletions near the C terminus produced only nonflagellate cells. On the basis of this evidence and the results of a previous study of spontaneous FliM mutants (H. Sockett, S. Yamaguchi, M. Kihara, V. M...Continue Reading

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·N R FrancisR M Macnab
Feb 1, 1992·Journal of Bacteriology·H SockettR M Macnab
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R B BourretM I Simon
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Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M WelchM Eisenbach
Aug 1, 1996·Journal of Bacteriology·M KiharaR M Macnab
Aug 16, 1996·Journal of Molecular Biology·H TangD F Blair

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Citations

May 22, 2001·Molecular Microbiology·R Barak, M Eisenbach
Sep 10, 2014·Journal of Bacteriology·Clive S BarkerFadel A Samatey
Dec 23, 2015·Molecular Microbiology·Clive S BarkerFadel A Samatey
Oct 21, 2016·Scientific Reports·Young-Ho YoonFadel A Samatey
Oct 26, 2005·The Journal of Biological Chemistry·Hedda U FerrisRobert M Macnab
Oct 14, 2010·PLoS Genetics·Clive S BarkerFadel A Samatey
Mar 24, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Tatsuro NishikinoMichio Homma
Nov 21, 2018·Journal of Bacteriology·Elizabeth WardDavid F Blair
Aug 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sang-Youn ParkBrian R Crane
Sep 2, 2003·Journal of Bacteriology·Gillian M FraserRobert M Macnab
Apr 3, 2004·Journal of Bacteriology·John S Van ArnamRobert M Macnab
May 16, 2000·Journal of Bacteriology·M KiharaR M Macnab
Apr 5, 2005·Journal of Bacteriology·Perry N BrownDavid F Blair
Oct 29, 1998·Journal of Bacteriology·M A MathewsD F Blair
Feb 7, 1998·Biochimica Et Biophysica Acta·S Khan
Aug 28, 2007·Biochimica Et Biophysica Acta·Dmitry Apel, Michael G Surette
Sep 28, 2006·Biochemistry·Jonathan L McMurryBertha González-Pedrajo

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