Deletion in the Z-line region of the titin gene in a baby hamster kidney cell line, BHK-21-Bi

FEBS Letters
M JäckelH Jockusch

Abstract

The gene for titin, a 4MDa myofibrillar protein, was analysed in golden hamster DNAs from different sources, using human cDNA probes and PCR. In the DNA from the BHK-21-Bi subline of baby hamster kidney cells, extended sequences coding for Z-line associated domains were missing, indicating a deletion that renders titin non-functional. These sequences were present in the original BHK-21 line and in hamster DNAs. Our finding shows that, due to the absence of selective pressure on a gene's function, genomic deterioration can occur in a permanent cell line and can lead to a loss of overlapping DNA stretches in both autosomes.

References

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Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
May 1, 1994·Biophysical Chemistry·K Maruyama
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Citations

Jun 27, 1998·Journal of Peptide Science : an Official Publication of the European Peptide Society·M RenilM Meldal
May 23, 1998·The Journal of Cell Biology·C MachadoD J Andrew
Aug 18, 2021·Journal of Cell Science·Ariane BiquandIsabelle Richard

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