PMID: 3753584Jan 17, 1986Paper

Deletion mapping of Sindbis virus DI RNAs derived from cDNAs defines the sequences essential for replication and packaging

Cell
R LevisS Schlesinger

Abstract

Defective-interfering (DI) genomes of a virus contain sequence information essential for their replication and packaging. They need not contain any coding information and therefore are a valuable tool for identifying cis-acting, regulatory sequences in a viral genome. To identify these sequences in a DI genome of Sindbis virus, we cloned a cDNA copy of a complete DI genome directly downstream of the promoter for the SP6 bacteriophage DNA dependent RNA polymerase. The cDNA was transcribed into RNA, which was transfected into chicken embryo fibroblasts in the presence of helper Sindbis virus. After one to two passages the DI RNA became the major viral RNA species in infected cells. Data from a series of deletions covering the entire DI genome show that only sequences in the 162 nucleotide region at the 5' terminus and in the 19 nucleotide region at the 3' terminus are specifically required for replication and packaging of these genomes.

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Citations

Sep 5, 2002·Reviews in Medical Virology·Jonathan O RaynerKurt I Kamrud
Oct 1, 1993·Trends in Microbiology·C R Bangham, T B Kirkwood
Jul 22, 1998·Journal of Virological Methods·M M Nagarajan, F S Kibenge
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J TartagliaD L Nuss
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R LevisS Schlesinger
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J V AnzolaD L Nuss
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B Mirakhur, R W Peluso
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·U Geigenmüller-GnirkeS Schlesinger
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Jan 30, 2013·PLoS Neglected Tropical Diseases·Kali D Saxton-ShawAnn M Powers
Jun 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Hung-Yi Wu, David A Brian
Jul 9, 2014·Proceedings of the National Academy of Sciences of the United States of America·Dal Young KimIlya Frolov

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