PMID: 6402710Feb 17, 1983Paper

Deletion mapping of the inducible promoter of human IFN-beta gene

Nature
J TavernierW Fiers

Abstract

Human fibroblast cells produce beta-type interferon only in response to viral infection or treatment with an inducer such as poly(rI) X poly(rC); this event is most probably controlled at the transcriptional level (for review see ref. 1). To study the induction process, we inserted the human fibroblast interferon (IFN-beta) gene, with or without its promoter region, into recombinant simian virus 40 (SV40) plasmid vectors which subsequently were transfected into monkey AP-8 cells. We report here that upon induction with poly(rI) X poly(rC) there was a 10-30-fold increase in IFN-beta synthesis. This inducer had no effect on interferon production when the coding region only was inserted into the vector plasmid, which indicates that the promoter region is required for inducibility of this gene. Deletion mapping implicates the region between nucleotides -144 and -186 from the mRNA initiation site in the specific regulation of the IFN-beta gene. This region contains a sequence that is remarkably homologous with a consensus sequence found in the 5' flanking region of steroid hormone responsive genes, which might be involved in binding the progesterone-receptor complex.

References

Sep 25, 1980·Nucleic Acids Research·D V GoeddelS Pestka
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T TaniguchiM Muramatsu
Jan 24, 1981·Nucleic Acids Research·M HoughtonN H Carey
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S MaedaS Pestka
Mar 11, 1981·Nucleic Acids Research·R M LawnD V Goeddel
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R M LawnD V Goeddel
Aug 26, 1982·Nature·P W Gray, D V Goeddel
Sep 24, 1982·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·W FiersJ Content
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K ZinnT Maniatis
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D Canaani, P Berg
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S Ohno, T Taniguchi

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Citations

May 1, 1991·Journal of Cellular Biochemistry·D S HainesD H Gillespie
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M RingoldF McCormick
Jan 1, 1985·Journal of Interferon Research·N B RajP M Pitha
May 11, 1984·Nucleic Acids Research·J HiscottC Weissmann
Jul 1, 1987·European Journal of Biochemistry·H Dinter, H Hauser
Jan 1, 1984·Molecular and Cellular Biology·F McCormickG Ringold

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