PMID: 9525608Apr 3, 1998Paper

Deletion of a CD2-like gene, 8-DR, from African swine fever virus affects viral infection in domestic swine

Journal of Virology
M V BorcaD L Rock

Abstract

An African swine fever virus (ASFV) gene with similarity to the T-lymphocyte surface antigen CD2 has been found in the pathogenic African isolate Malawi Lil-20/1 (open reading frame [ORF] 8-DR) and a cell culture-adapted European virus, BA71V (ORF EP402R) and has been shown to be responsible for the hemadsorption phenomenon observed for ASFV-infected cells. The structural and functional similarities of the ASFV gene product to CD2, a cellular protein involved in cell-cell adhesion and T-cell-mediated immune responses, suggested a possible role for this gene in tissue tropism and/or immune evasion in the swine host. In this study, we constructed an ASFV 8-DR gene deletion mutant (delta8-DR) and its revertant (8-DR.R) from the Malawi Lil-20/1 isolate to examine gene function in vivo. In vitro, delta8-DR, 8-DR.R, and the parental virus exhibited indistinguishable growth characteristics on primary porcine macrophage cell cultures. In vivo, 8-DR had no obvious effect on viral virulence in domestic pigs; disease onset, disease course, and mortality were similar for the mutant delta8-DR, its revertant 8-DR.R, and the parental virus. Altered viral infection was, however, observed for pigs infected with delta8-DR. A delay in spread to a...Continue Reading

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Citations

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Jan 17, 2008·The Journal of General Virology·David A G ChapmanLinda K Dixon
Jan 21, 2011·Journal of Virology·Lynnette C Goatley, Linda K Dixon
Apr 8, 2011·Emerging Infectious Diseases·David A G ChapmanLinda K Dixon
Dec 20, 2014·The Journal of General Virology·A MalogolovkinD L Rock
Mar 11, 2020·Frontiers in Veterinary Science·Huldah SangWaithaka Mwangi
Aug 18, 2017·Journal of Virology·Paula L MonteagudoFernando Rodríguez
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Jun 2, 2020·Frontiers in Veterinary Science·Natasha N GaudreaultJuergen A Richt
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