Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn(2+)/H(+) antiporter of Arabidopsis thaliana, stimulates the transport activity.

The Journal of Biological Chemistry
Miki KawachiMasayoshi Maeshima

Abstract

Arabidopsis thaliana AtMTP1 belongs to the cation diffusion facilitator family and is localized on the vacuolar membrane. We investigated the enzymatic kinetics of AtMTP1 by a heterologous expression system in the yeast Saccharomyces cerevisiae, which lacked genes for vacuolar membrane zinc transporters ZRC1 and COT1. The yeast mutant expressing AtMTP1 heterologously was tolerant to 10 mm ZnCl(2). Active transport of zinc into vacuoles of living yeast cells expressing AtMTP1 was confirmed by the fluorescent zinc indicator FuraZin-1. Zinc transport was quantitatively analyzed by using vacuolar membrane vesicles prepared from AtMTP1-expressing yeast cells and radioisotope (65)Zn(2+). Active zinc uptake depended on a pH gradient generated by endogenous vacuolar H(+)-ATPase. The activity was inhibited by bafilomycin A(1), an inhibitor of the H(+)-ATPase. The K(m) for Zn(2+) and V(max) of AtMTP1 were determined to be 0.30 microm and 1.22 nmol/min/mg, respectively. We prepared a mutant AtMTP1 that lacked the major part (32 residues from 185 to 216) of a long histidine-rich hydrophilic loop in the central part of AtMTP1. Yeast cells expressing the mutant became hyperresistant to high concentrations of Zn(2+) and resistant to Co(2+). T...Continue Reading

References

Jan 1, 1991·Advances in Protein Chemistry·D W Christianson
Jan 1, 1991·Methods in Enzymology·E W Jones
Feb 14, 1990·European Journal of Biochemistry·C Matsuura-EndoS Yoshida
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E J BowmanK Altendorf
Mar 15, 1997·The Journal of Membrane Biology·I T Paulsen, M H Saier
Apr 5, 2000·Biochimica Et Biophysica Acta·M Maeshima
Apr 5, 2000·Biochimica Et Biophysica Acta·L E WilliamsJ L Hall
Apr 5, 2000·Biochimica Et Biophysica Acta·M L Guerinot
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·M W PersansD E Salt
Aug 6, 2002·The Journal of Biological Chemistry·Colin W MacDiarmidDavid J Eide
Jan 31, 2003·The Journal of Biological Chemistry·Colin W MacDiarmidDavid J Eide
Apr 16, 2005·Current Opinion in Biotechnology·Ute Krämer
Jun 11, 2005·Trends in Plant Science·Ute Krämer
Sep 1, 2005·Journal of Experimental Botany·Masakazu HaraToru Kuboi
Sep 13, 2005·Trends in Plant Science·Lorraine E Williams, Rebecca F Mills
Jan 11, 2007·Trends in Biochemical Sciences·Roland GamsjaegerJoel P Mackay
Apr 7, 2007·FEBS Letters·Roberto A GaxiolaKarin Schumacher
Aug 25, 2007·Science·Min Lu, Dax Fu

❮ Previous
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Citations

Jan 24, 2009·European Biophysics Journal : EBJ·Shira CoremJoachim Scholz-Starke
Dec 11, 2008·Environmental Science and Pollution Research International·Abdul R Memon, Peter Schröder
Jun 11, 2011·Biochemistry·Murli ManoharKendal D Hirschi
Apr 16, 2009·The Journal of Biological Chemistry·Ehud OhanaIsrael Sekler
Dec 6, 2011·The Journal of Biological Chemistry·Dorina PodarDale Sanders
Jun 14, 2013·Journal of Experimental Botany·Paloma K MenguerLorraine E Williams
Oct 13, 2009·Diabetes, Obesity & Metabolism·N WijesekaraM B Wheeler
Sep 22, 2009·Genome Génome / Conseil National De Recherches Canada·Melinda A Klein, Michael A Grusak
Aug 14, 2009·American Journal of Physiology. Cell Physiology·Haley B NaikDavid I Soybel
Jan 14, 2011·Bioscience, Biotechnology, and Biochemistry·Yukari MutoMasayoshi Maeshima
Mar 13, 2012·Annual Review of Plant Biology·Enrico MartinoiaRéka Nagy
Jul 19, 2013·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Isil ErbasolHuseyin Caglar Karakaya
Aug 21, 2012·Cellular and Molecular Life Sciences : CMLS·Ya-Fen Lin, Mark G M Aarts
Oct 21, 2015·The Plant Journal : for Cell and Molecular Biology·Magdalena MigockaTadeusz Marchewka
Oct 6, 2009·Plant Physiology and Biochemistry : PPB·Mingliang ChenTao Wang
Jun 2, 2009·Current Opinion in Plant Biology·Sergi Puig, Lola Peñarrubia
Sep 15, 2012·The Plant Journal : for Cell and Molecular Biology·Thomas SchneiderStefan Meyer
Sep 2, 2011·The Plant Journal : for Cell and Molecular Biology·Takanori KobayashiNaoko K Nishizawa
Jan 6, 2016·Plant, Cell & Environment·Shanti S SharmaTetsuro Mimura
May 1, 2012·Journal of Environmental Management·Atul BhargavaShilpi Srivastava
Nov 26, 2014·Journal of Experimental Botany·Magdalena MigockaSophie Filleur
Jun 15, 2014·Journal of Experimental Botany·Nadia BazihizinaStefano Mancuso
Jul 1, 2017·Metallomics : Integrated Biometal Science·Yarden GolanYehuda G Assaraf
Jun 13, 2017·Metallomics : Integrated Biometal Science·Hualiang Pi, John D Helmann
Apr 21, 2018·Plant, Cell & Environment·Chun-Lin ChenHong-Qing Ling
Jun 23, 2018·The Plant Journal : for Cell and Molecular Biology·Magdalena MigockaArnold Garbiec
May 31, 2018·Antimicrobial Agents and Chemotherapy·Richard J WallSusan Wyllie
Nov 13, 2010·Metallomics : Integrated Biometal Science·Claudia A Blindauer, Ralf Schmid
May 14, 2011·Metallomics : Integrated Biometal Science·Toshiyuki Fukada, Taiho Kambe
Oct 1, 2013·Metallomics : Integrated Biometal Science·Ciro CubillasAlejandro García-de los Santos
Aug 1, 2019·Critical Reviews in Biotechnology·Kanchan VishwakarmaShivesh Sharma

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