Deletion of amino acids Glu146-->Arg160 in human apolipoprotein A-I (ApoA-ISeattle) alters lecithin:cholesterol acyltransferase activity and recruitment of cell phospholipid

Biochemistry
E M LindholmT M Forte

Abstract

Human apolipoprotein A-I (apoA-I) has an important role in the efflux of cholesterol from peripheral cells, the first step in reverse cholesterol transport. Deletion of amino acids Glu146-->Arg160 in apoA-I (apoA-ISeattle) removes a large section of a lipid binding helix and is associated in vivo with an atherogenic lipoprotein profile characterized by a deficiency in high-density lipoproteins (HDL). In the present study, we asked whether apoA-ISeattle had normal ability to recruit lipids from cells and to form nascent high-density lipoprotein (HDL) particles and whether the altered secondary structure affected lecithin:cholesterol acyltransferase (LCAT) activity. Wild-type apoA-I and apoA-ISeattle expressed in transfected Chinese hamster ovary cells formed nascent HDL particles with similar density distribution and protein-to-lipid ratio. Phospholipid subclass distribution of apoA-ISeattle nascent HDL demonstrated a significant increase in sphingomyelin and phosphatidylethanolamine compared to wild type. ApoA-ISeattle nascent HDL had a unique size distribution compared to wild-type nascent HDL; large (9-20 nm) particles predominated while there were virtually no small (7.5 nm) particles. LCAT reactivity was impaired by apoA-IS...Continue Reading

Citations

Sep 23, 2000·Chemistry and Physics of Lipids·J P Slotte
May 15, 2002·Trends in Cardiovascular Medicine·Mary G Sorci-Thomas, Michael J Thomas
Mar 30, 2001·Biochimica Et Biophysica Acta·C G BrouilletteD W Borhani
Nov 13, 1998·Current Opinion in Lipidology·M NavabA M Fogelman
Nov 5, 1999·Protein Expression and Purification·D SviridovN Fidge
Oct 7, 2004·Journal of the American College of Cardiology·G Kees HovinghJohn J P Kastelein
Oct 28, 2005·Journal of Lipid Research·Ake Nilsson, Rui-Dong Duan
Apr 11, 2013·International Journal of Molecular Sciences·Roberto Martínez-BeamonteJesús Osada
Mar 14, 2018·Current Medicinal Chemistry·Angeliki Chroni, Dimitris Kardassis
Nov 10, 2004·The Journal of Biological Chemistry·Maria Stefania SpagnuoloPaolo Abrescia
May 16, 2006·The Journal of Biological Chemistry·Dale D O MartinMichael N Oda
Sep 19, 2002·Biochemical and Biophysical Research Communications·Zhen JiaJohn K Bielicki

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