PMID: 7544312Aug 1, 1995Paper

Deletion of beta 1 integrins in mice results in inner cell mass failure and peri-implantation lethality

Genes & Development
L E StephensC H Damsky

Abstract

Integrin receptors for extracellular matrix receptors are important effectors of cell adhesion, differentiation, and migration in cultured cells and are believed to be critical effectors of these processes during development. To determine when beta 1 integrins become critical during embryonic development, we generated mutant mice with a targeted disruption of the beta 1 integrin subunit gene. Heterozygous mutant mice were normal. Homozygous loss of beta 1 integrin expression was lethal during early postimplantation development. Homozygous embryos lacking beta 1 integrins formed normal-looking blastocysts and initiated implantation at E4.5. However, the E4.5 beta 1-null embryos in situ had collapsed blastocoeles, and whereas the trophoblast penetrated the uterine epithelium, extensive invasion of the decidua was not observed. Laminin-positive endoderm cells were detected in the inner cell mass area, but endoderm morphogenesis and migration were defective. By E5.5 beta 1-null embryos had degenerated extensively. In vitro analysis showed that trophoblast function in beta 1-null peri-implantation embryos was largely normal, including expression of tissue-specific markers, and outgrowth on fibronectin- and vitronectin-coated, althou...Continue Reading

References

Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D Solter, B B Knowles
Aug 1, 1994·Current Opinion in Genetics & Development·R O Hynes
Aug 1, 1995·Genes & Development·R Fässler, M Meyer
Jan 13, 1995·Science·B FeldmanM Goldfarb
Apr 1, 1994·Molecular Biology of the Cell·M A Schwartz, D E Ingber
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·L LarueR Kemler
Sep 22, 1994·Nature·F GuillemotA L Joyner
May 20, 1994·Cell·E Ruoslahti, J C Reed
Nov 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C DamskyS Fisher
Oct 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J M BreussR Pytela
Sep 1, 1993·Molecular Biology of the Cell·J E MeredithM A Schwartz
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S A WoodN D Allen

❮ Previous
Next ❯

Citations

Aug 26, 1998·Cell Motility and the Cytoskeleton·C L Moncman, K Wang
Jun 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H Colognato, P D Yurchenco
Jan 1, 1997·Developmental Genetics·J L RinkenbergerZ Werb
Jul 11, 2000·The Journal of Comparative Neurology·H HeringS Kröger
Oct 2, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J M LoraK S Zaret
Apr 2, 2009·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Masahiro Murakami, Michael Simons
Nov 15, 2008·Histochemistry and Cell Biology·Cyrus M Ghajar, Mina J Bissell
Nov 1, 2005·Cancer Metastasis Reviews·C Brakebusch, R Fässler
Feb 27, 2004·Biochimica Et Biophysica Acta·Curzio RüeggAgnese Mariotti
Apr 20, 2004·The International Journal of Biochemistry & Cell Biology·Richard P Tucker
Mar 5, 2004·Matrix Biology : Journal of the International Society for Matrix Biology·Peter D YurchencoBruce L Patton
Apr 20, 2005·Neurobiology of Disease·Hiroki KurahashiTatsushi Toda
Aug 13, 1998·Developmental and Comparative Immunology·A L Medvinsky, E A Dzierzak
May 14, 1998·Neuroscience and Biobehavioral Reviews·R J Nelson, K A Young
Sep 6, 2000·Trends in Genetics : TIG·A De Arcangelis, E Georges-Labouesse
Apr 13, 2000·Biology of the Cell·T DarribèreD Alfandari
Mar 16, 2001·Progress in Neurobiology·S C PrevitaliH Hartung
Mar 1, 1997·Reproductive Toxicology·Ch DamskyS Fisher
Aug 11, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·D Sheppard
Jun 15, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·M Aumailley, P Rousselle
Oct 1, 1996·Current Opinion in Cell Biology·R FässlerE Hirsch
Oct 6, 1999·Current Opinion in Cell Biology·J E Schwarzbauer, J L Sechler
Oct 6, 1999·Current Opinion in Cell Biology·G BazzoniM G Lampugnani
Jul 18, 2002·BJOG : an International Journal of Obstetrics and Gynaecology·Andrew W HorneEl-Nasir Lalani
Mar 5, 2003·Trends in Molecular Medicine·Xuemei Tong, Pierre A Coulombe
Feb 24, 2012·Nature·Lingyan JinMichael Rape
Sep 3, 2002·Nature Medicine·Richard O Hynes
May 24, 2008·Nature Reviews. Cancer·Christie J AvraamidesJudith A Varner
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Kyle R LegateReinhard Fässler
Aug 17, 2012·Nature Reviews. Molecular Cell Biology·Georg HalderStefano Piccolo
Jun 8, 2001·The Biochemical Journal·A Ishii, S H Lo
Oct 3, 1998·Journal of Anatomy·M Aumailley, N Smyth
Mar 18, 2000·Proceedings of the Society for Experimental Biology and Medicine·J A Bowen, J S Hunt
May 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Xu PengJun-Lin Guan
Jul 30, 2009·The Journal of Biological Chemistry·Rosemary BassVincent Ellis
Jul 1, 1996·The Journal of Cell Biology·M D Martin-Bermudo, N H Brown
Sep 11, 2013·The Journal of Cell Biology·Daniel H MadsenThomas H Bugge

❮ Previous
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