Deletion of Cdc42 in embryonic cardiomyocytes results in right ventricle hypoplasia

Clinical and Translational Medicine
Yang LiuXu Peng

Abstract

Cdc42 is a member of the Rho GTPase family and functions as a molecular switch in regulating cytoskeleton remodeling and cell polarity establishment. Inactivating Cdc42 in cardiomyocytes resulted in embryonic lethality with heart developmental defects, including ventricular septum defects and thin ventricle wall syndrome. In this study, we have generated a Cdc42 cardiomyocyte knockout mouse line by crossing Cdc42/flox mice with myosin light chain 2a (MLC2a)-Cre mice. We found that the deletion of Cdc42 in embryonic cardiomyocytes resulted in an underdeveloped right ventricle. Microarray analysis and real-time PCR data analysis displayed that the deletion of Cdc42 decreased dHand expression level. In addition, we found evaginations in the ventricle walls of Cdc42 knockout hearts. We concluded that Cdc42 plays an essential role in right ventricle growth.

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Citations

Dec 3, 2021·Journal of Cellular and Molecular Medicine·Junming SunMin He

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Methods Mentioned

BETA
GTPase
nucleotide exchange
PCR
Chip

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