Deletion of ΔdblGata motif leads to increased predisposition and severity of IgE-mediated food-induced anaphylaxis response

PloS One
Sribava SharmaSimon P Hogan

Abstract

Previous studies have revealed an important role for the transcription factor GATA-1 in mast cell maturation and degranulation. However, there have been conflicting reports with respect to the requirement of GATA-1 function in mast cell dependent inflammatory processes. Herein, we examine the requirement of GATA-1 signaling in mast cell effector function and IgE-mast cell-dependent anaphylaxis. To study the requirement of GATA-1 dependent signaling in the development and severity of IgE-mast cell-dependent anaphylaxis in mice. Wild type (Balb/c) and mutant ΔdblGata (Balb/c) mice were employed to study the role of GATA-1 signaling in in vitro IgE-mediated activation of bone marrow derived mast cells (BMMCs). Murine models of passive IgE-mediated and oral antigen-induced IgE-mediated anaphylaxis were employed in mice. Frequency of steady state mast cells in various tissues (duodenum, ear, and tongue), peritoneal cavity, and clinical symptoms (diarrhea, shock, and mast cell activation) and intestinal Type 2 immune cell analysis including CD4+ Th2 cells, type 2 innate lymphoid cells (ILC2), and IL-9 secreting mucosal mast cells (MMC9) were assessed. In vitro analysis revealed that ΔdblGata BMMCs exhibit a reduced maturation rate, d...Continue Reading

References

Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y FujiwaraS H Orkin
May 9, 1997·The Journal of Biological Chemistry·S TakahashiM Yamamoto
Feb 9, 1999·The Journal of Allergy and Clinical Immunology·X M LiH A Sampson
Dec 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Keiko MaedaChisei Ra
Feb 5, 2003·The Journal of Experimental Medicine·Anna Rita MigliaccioStuart H Orkin
Mar 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Richard T StraitFred D Finkelman
Jul 4, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Junko WakabayashiMasayuki Yamamoto
Dec 9, 2003·The Journal of Clinical Investigation·Eric B BrandtMarc E Rothenberg
Sep 18, 2004·Science·Alison A HumblesCraig Gerard
Dec 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yojiro ArinobuKoichi Akashi
Aug 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Brian O BarnsteinJohn J Ryan
Sep 4, 2007·The Journal of Allergy and Clinical Immunology·Fred D Finkelman
Jan 4, 2008·The New England Journal of Medicine·Peter VadasJupiter Yeung
Apr 2, 2008·The Journal of Experimental Medicine·Elizabeth E ForbesSimon P Hogan
Sep 30, 2008·Current Opinion in Immunology·Donald MacGlashan
Nov 11, 2008·The Journal of Allergy and Clinical Immunology·Eric B BrandtMarc E Rothenberg
Dec 22, 2009·Future Microbiology·Michelle N Kelly, Judd E Shellito
Feb 18, 2010·The Journal of Allergy and Clinical Immunology·Heather OsterfeldSimon P Hogan
Jul 10, 2010·Lung India : Official Organ of Indian Chest Society·Sudha S DeoPramod V Niphadkar
Aug 6, 2010·Cell and Tissue Research·Yael Minai-FlemingerFrancesca Levi-Schaffer
Feb 18, 2011·Immunology·Shigeo Koyasu, Kazuyo Moro
Feb 11, 2012·The American Journal of Pathology·Richard AhrensSimon P Hogan
May 9, 2012·Nature Medicine·Stephen J Galli, Mindy Tsai
Jul 25, 2012·Allergology International : Official Journal of the Japanese Society of Allergology·Yosuke BabaChiharu Nishiyama
Aug 29, 2012·Seminars in Immunopathology·Simon P HoganFred D Finkelman
Feb 5, 2013·The Journal of Allergy and Clinical Immunology·Lisa M BartnikasMichiko K Oyoshi
May 2, 2013·The Journal of Allergy and Clinical Immunology·Marat V KhodounFred D Finkelman
Aug 7, 2013·The Journal of Allergy and Clinical Immunology·Simon G A BrownGeoffrey K Isbister
Sep 18, 2013·JAMA Pediatrics·Ruchi GuptaDavid Meltzer
Oct 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yuichiro NeiHajime Karasuyama
Mar 13, 2014·Molecular and Cellular Biology·Kinuko OhnedaMasayuki Yamamoto
Mar 19, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eisuke InageChiharu Nishiyama

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Citations

Oct 11, 2019·International Journal of Molecular Sciences·Misun KimDooil Jeoung
Dec 19, 2020·Frontiers in Immunology·Kazushige Obata-NinomiyaSteven F Ziegler
Mar 2, 2021·Annual Review of Immunology·Elizabeth A JacobsenMarc E Rothenberg

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Methods Mentioned

BETA
FACS
flow cytometry
transgenic
lavage

Software Mentioned

Prism
GraphPad
Flowjo
Image Processing

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