Deletion of ENTPD3 does not impair nucleotide hydrolysis in primary somatosensory neurons or spinal cord

F1000Research
Eric McCoyMark J Zylka

Abstract

Ectonucleotidases are membrane-bound or secreted proteins that hydrolyze extracellular nucleotides.  Recently, we identified three ectonucleotidases that hydrolyze extracellular adenosine 5'-monophosphate (AMP) to adenosine in primary somatosensory neurons.  Currently, it is unclear which ectonucleotidases hydrolyze ATP and ADP in these neurons.  Ectonucleoside triphosphate diphosphohydrolases (ENTPDs) comprise a class of enzymes that dephosphorylate extracellular ATP and ADP.  Here, we found that ENTPD3 (also known as NTPDase3 or CD39L3) was located in nociceptive and non-nociceptive neurons of the dorsal root ganglion (DRG), in the dorsal horn of the spinal cord, and in free nerve endings in the skin.  To determine if ENTPD3 contributes directly to ATP and ADP hydrolysis in these tissues, we generated and characterized an Entpd3 knockout mouse.  This mouse lacks ENTPD3 protein in all tissues examined, including the DRG, spinal cord, skin, and bladder.  However, DRG and spinal cord tissues from Entpd3 (-/-) mice showed no reduction in histochemical staining when ATP, ADP, AMP, or UTP were used as substrates.  Additionally, using fast-scan cyclic voltammetry (FSCV), adenosine production was not impaired in the dorsal spinal cor...Continue Reading

References

Sep 15, 1996·Analytical Chemistry·P S CahillR M Wightman
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·F Nakamura, S M Strittmatter
Jul 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Gregory ArcuinoMaiken Nedergaard
Jun 6, 2003·Progress in Neurobiology·Jana Sawynok, Xue Jun Liu
Oct 29, 2003·The Journal of Biological Chemistry·Rolf WesterbergAnders Jacobsson
Mar 24, 2004·The Journal of Biological Chemistry·Tokuyuki ShinoharaShuji Hinuma
May 8, 2004·Biochemical Pharmacology·Elise G LavoieJean Sévigny
Jun 15, 2004·Nature Medicine·Makoto InoueHiroshi Ueda
Jan 26, 2005·Trends in Neurosciences·Makoto TsudaMichael W Salter
Nov 8, 2006·Handbook of Experimental Pharmacology·J Sawynok
Jan 16, 2007·Analytical Chemistry·B E Kumara Swamy, B Jill Venton
Apr 13, 2007·Physiological Reviews·Geoffrey Burnstock
May 8, 2007·Neuron·Ajay DhakaArdem Patapoutian
Jul 3, 2007·British Journal of Pharmacology·S A LévesqueJ Sévigny
Apr 12, 2008·Purinergic Signalling·F KukulskiJ Sévigny
Dec 26, 2008·Molecular Pain·Yoshizo MatsukaIgor Spigelman
Jan 28, 2009·Brain Research Reviews·G DussorD C Molliver
Jul 3, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kristofer K RauH Richard Koerber
Oct 31, 2009·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Pietro CiancagliniJosé Luis Millán
Jan 23, 2010·Molecular Pain·Clive GentryStuart Bevan
Feb 12, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nathaniel A SowaMark J Zylka
Jun 18, 2010·European Journal of Pharmacology·Joanna Ripoll RoziskyIraci Lucena da Silva Torres
Jul 22, 2010·Science·Alexander V GourineSergey Kasparov
Aug 5, 2010·American Journal of Physiology. Endocrinology and Metabolism·Elise G LavoieJean Sévigny
Aug 6, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nathaniel A SowaMark J Zylka
Jan 15, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Elise G LavoieJean Sévigny
Jan 18, 2011·Trends in Molecular Medicine·Mark J Zylka
Nov 11, 2011·Journal of Neurophysiology·Sheldon R GarrisonCheryl L Stucky
Jun 30, 2012·PloS One·Daniel S BrennerRobert W Gereau
Oct 19, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Qin LiuXinzhong Dong
Jul 5, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sarah E StreetMark J Zylka

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Citations

May 27, 2015·Autonomic Neuroscience : Basic & Clinical·Andréia Machado CardosoJean Sévigny
Oct 16, 2014·Nature Reviews. Neuroscience·Paul A Howard-Jones

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