Deletion of Epstein-Barr virus latent membrane protein 1 gene in United States and Brazilian Hodgkin's disease and reactive lymphoid tissue: high frequency of a 30-bp deletion

Human Pathology
K HayashiL M Weiss

Abstract

A 30-basepair (bp) deletion in the Epstein-Barr virus (EBV) latent membrane protein 1 (LMP1) gene has been reported in nasopharyngeal carcinoma and EBV-associated malignant lymphomas. Prior studies have found the deletion in about 10% to 28% of cases of Hodgkin's disease (HD), particularly in cases with aggressive histology. We studied the prevalence of 30-bp LMP1 gene deletion in EBV-positive HD in the United States (US) (12 cases) and Brazil (26 cases) with comparison to reactive lymphoid tissues (21 cases) and HD without EBV-positive Reed-Sternberg cells (15 cases). We studied the status of the LMP1 gene by Southern blot hybridization of polymerase chain reaction (PCR) products obtained after amplification with primers spanning the site of the deletion. We also performed EBV typing, EBER1 in situ hybridization, and LMP1 protein immunohistochemistry. EBV was detected in 12/26 (46%) cases of HD from the US and 26/27 (96%) cases of Brazilian HD. The 30-bp LMP1 gene deletion was observed in 4/12 (33%) cases of EBV-positive HD from US, and 12/26 (46%) cases of Brazilian EBV-positive HD, including 3 cases of type B EBV, as compared with 12/21 (57%) reactive lymphoid tissues and 9/15 (60%) cases of EBV-negative HD. US and Brazilian...Continue Reading

References

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Citations

Mar 5, 2008·Cancer Research·Tianli XiaWilliam J Harrington
Oct 18, 2014·Chinese Journal of Cancer·Yu Du, Jennifer R Grandis
Dec 17, 2008·Forensic Science International. Genetics·Hiroshi Ikegaya
Dec 21, 2000·Current Oncology Reports·L M Weiss
Mar 29, 2002·Journal of Medical Virology·Insun KimYoung-Sik Kim
Jan 23, 1999·Current Opinion in Hematology·D E Mosier
Jul 15, 1999·International Journal of Cancer. Journal International Du Cancer·M F EssopG Pallesen

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