Deletion of Invasion Protein B in Salmonella enterica Serovar Typhimurium Influences Bacterial Invasion and Virulence

Current Microbiology
Songbiao ChenYang Wang

Abstract

Salmonella enterica serovar Typhimurium (S. Typhimurium) has a wide host range and causes infections ranging from severe gastroenteritis to systemic infections in human, as well as causing typhoid-like disease in murine models of infection. S. Typhimurium translocates its effector proteins through the Salmonella pathogenicity island-I (SPI-I)-encoded T3SS-I needle complex. This study focuses on invasion protein B (SipB) of S. Typhimurium, which plays an active role in SPI-I invasion efficiency. To test our hypothesis, a sipB deletion mutant was constructed through double-crossover allelic using the suicide vector pRE112ΔsipB, and its biological characteristics were analyzed. The results showed that the SipB does not affect the growth of Salmonella, but the adherence, invasion, and virulence of the mutant were significantly decreased compared with wild-type S. Typhimurium (SL1344). This research indicates that SipB is an important virulence factor in the pathogenicity of S. Typhimurium.

References

May 29, 1998·Infection and Immunity·A W van der VeldenF Heffron
Aug 26, 1998·Infection and Immunity·J M ReyratR Rappuoli
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·D HershA Zychlinsky
Dec 1, 1999·Cell Death and Differentiation·H R Stennicke, G S Salvesen
Oct 8, 2004·Cellular Microbiology·Karsten Hueffer, Jorge E Galán
Sep 6, 2005·Trends in Microbiology·Siegfried Hapfelmeier, Wolf-Dietrich Hardt
Sep 15, 2012·Biochemical and Biophysical Research Communications·Hiroshi AsakuraShigeki Yamamoto

❮ Previous
Next ❯

Citations

Mar 19, 2021·FEMS Microbiology Reviews·Amber J BartonAndrew J Pollard

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.