Deletion of maize RDM4 suggests a role in endosperm maturation as well as vegetative and stress-responsive growth.

Journal of Experimental Botany
Shangang JiaDavid R Holding

Abstract

Opaque kernels in maize may result from mutations in many genes, such as OPAQUE-2. In this study, a maize null mutant of RNA-DIRECTED DNA METHYLATION 4 (RDM4) showed an opaque kernel phenotype, as well as plant developmental delay, male sterility, and altered response to cold stress. We found that in opaque kernels, all zein proteins were reduced and amino acid content was changed, including increased lysine. Transcriptomic and proteomic analysis confirmed the zein reduction and proteomic rebalancing of non-zein proteins, which was quantitatively and qualitatively different from opaque-2. Global transcriptional changes were found in endosperm and leaf, including many transcription factors and tissue-specific expressed genes. Furthermore, of the more than 8000 significantly differentially expressed genes in wild type in response to cold, a significant proportion (25.9% in moderate cold stress and 40.8% in near freezing stress) were not differentially expressed in response to cold in rdm4, suggesting RDM4 may participate in regulation of abiotic stress tolerance. This initial characterization of maize RDM4 provides a basis for further investigating its function in endosperm and leaf, and as a regulator of normal and stress-respon...Continue Reading

References

Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R J SchmidtB Burr
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·J E HabbenB A Larkins
Aug 13, 2008·Plant Biotechnology Journal·Monica A Schmidt, Eliot M Herman
Aug 9, 2011·Journal of Agricultural and Food Chemistry·Laura S da SilvaJohn R N Taylor
Apr 3, 2012·Expert Review of Proteomics·Feng YangRichard D Smith
Jun 10, 2014·Frontiers in Plant Science·Yongrui Wu, Joachim Messing
Jun 28, 2014·Genome Biology·Ryan M LayerIra M Hall
Jul 8, 2014·Frontiers in Plant Science·David R Holding
Dec 11, 2014·Annual Review of Plant Biology·Marjori A MatzkeAntonius J M Matzke
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
May 6, 2015·Current Genomics·Meng Xie, Bin Yu
Jul 18, 2015·Plant Cell Reports·Ali MovahediQiang Zhuge
Sep 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Zhiyong ZhangYongrui Wu
Feb 6, 2017·Journal of Experimental Botany·Huang HuangSusheng Song
Oct 8, 2017·Methods in Molecular Biology·Shangang JiaDavid Holding
Nov 9, 2018·Current Protocols in Plant Biology·Abou Yobi, Ruthie Angelovici

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.