Deletion of myosin light chain kinase in endothelial cells has a minor effect on the lipopolysaccharide-induced increase in microvascular endothelium permeability in mice

The FEBS Journal
Yang YuHua-Qun Chen

Abstract

There is a current view that myosin light chain kinase (MLCK) plays a critical role in endothelial permeability. To investigate the functions of MLCK in endothelial cells in vivo, we generated a mouse model in which MLCK was selectively deleted by crossing Mylk1 floxed mice with Tie2/cre transgenic mice. Knocking out Mylk1 from endothelial cells had no effect on the global phenotype of the mice, including body weight and blood pressure. Lipopolysaccharide (LPS)-mediated septic death was also not altered in the knockout (KO) mice. Consistently, LPS-induced inflammatory injury and the increase in microvascular permeability in the main organs, including the lung and the kidney, was not significantly attenuated in KO mice as compared with wild-type mice. However, the LPS-induced microvascular hyperpermeability of the esophagus and the eyeballs was attenuated in KO mice. We also found that the LPS-mediated increase in the number of caveolae in the endothelial cells of the esophagus was significantly reduced in KO mice. Our results do not support a role for endothelial cell MLCK in the pathogenesis of inflammatory diseases.

References

Dec 8, 1995·The Journal of Biological Chemistry·P J GallagherB P Herring
Nov 17, 1994·Nature·A P Somlyo, A V Somlyo
Jan 1, 1996·Seminars in Thrombosis and Hemostasis·J G GarciaK L Schaphorst
Nov 7, 1998·American Journal of Respiratory Cell and Molecular Biology·A D VerinJ G Garcia
Jan 15, 1999·Acta Physiologica Scandinavica·J T StullG Zhi
May 10, 2000·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·C W Lush, P R Kvietys
Sep 26, 2000·American Journal of Physiology. Cell Physiology·J H TinsleyS Y Yuan
Nov 30, 2000·The Journal of Biological Chemistry·K E Kamm, J T Stull
Feb 13, 2001·Developmental Biology·Y Y KisanukiM Yanagisawa
Jun 22, 2001·Critical Reviews in Biomedical Engineering·A L Baldwin, G Thurston
Nov 2, 2001·The Journal of Biological Chemistry·W SchubertM P Lisanti
Feb 8, 2002·American Journal of Physiology. Cell Physiology·Emily K BluePatricia J Gallagher
May 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mark S WainwrightD Martin Watterson
Aug 2, 2003·Journal of Cellular Physiology·Carine Michiels
Mar 17, 2004·American Journal of Respiratory and Critical Care Medicine·Xinqi PengJoe G N Garcia
Sep 27, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kayo Miyawaki-ShimizuAsrar B Malik
Sep 27, 2005·The Journal of Clinical Investigation·Daniel R ClayburghJerrold R Turner
Dec 24, 2005·Physiological Reviews·Dolly Mehta, Asrar B Malik
Jan 13, 2006·Laboratory Investigation; a Journal of Technical Methods and Pathology·Stephanie A BlairJerrold R Turner
Feb 8, 2006·Annual Review of Physiology·Karnam S Murthy
Jun 16, 2006·American Journal of Physiology. Cell Physiology·B Paul HerringJiliang Zhou
Feb 20, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Janet L RossiMark S Wainwright
Apr 1, 2008·Annals of the New York Academy of Sciences·Emily VandenbrouckeAsrar B Malik
Nov 7, 2009·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M C CichyM H Bellini
Feb 13, 2010·Annual Review of Physiology·Yulia Komarova, Asrar B Malik

❮ Previous
Next ❯

Citations

Jul 25, 2013·Cell and Tissue Research·Ken KobayashiHaruto Kumura
Mar 21, 2013·Science Signaling·Arti V ShindeMohamed Trebak
Feb 13, 2013·Annual Review of Physiology·Jahar Bhattacharya, Michael A Matthay
Oct 16, 2015·International Immunopharmacology·Han LiuJunping Kou
May 20, 2014·The International Journal of Biochemistry & Cell Biology·Yu WangMin-Sheng Zhu
Mar 7, 2017·Biochemistry. Biokhimii︠a︡·A Y Khapchaev, V P Shirinsky
Apr 1, 2017·Military Medical Research·Chen-Yang DuanLiang-Ming Liu
Jun 11, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yiteng ZhangHuaqun Chen
Oct 24, 2013·The Journal of Biological Chemistry·Meng ChenB Paul Herring
Feb 6, 2017·Cellular and Molecular Life Sciences : CMLS·Michael SchnoorAlexander Zarbock
Jan 23, 2019·The Journal of Clinical Investigation·Sam C NalleJerrold R Turner
Aug 11, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nityanand SrivastavaDolly Mehta
Jul 5, 2017·European Journal of Trauma and Emergency Surgery : Official Publication of the European Trauma Society·J GrevenH C Pape

❮ Previous
Next ❯

Related Concepts

Related Feeds

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.