Abstract
The major bifunctional transpeptidases/transglycosylases of Escherichia coli, penicillin-binding proteins (PBPs) 1a and 1b, were evaluated for their influence on biofilm formation. While the PBP1a mutant was unaffected, the PBP1b mutant exhibited significantly decreased biofilm formation and motility. Interestingly, the extracellular indole concentration was higher in the PBP1b mutant, and similar phenotypic defects were replicated in the wild-type upon addition of exogenous indole. Expression of PBP1b in trans substantially decreased indole production and restored normal phenotypes. Results further suggest that rpoS deletion has a counteracting effect on the mrcB mutant. These findings indicate that PBP1b deletion influences biofilm formation and motility, possibly through indole.
References
Oct 29, 1998·Molecular Microbiology·L A Pratt, R Kolter
Jun 22, 2001·Journal of Bacteriology·D WangP N Rather
May 11, 2005·Molecular Microbiology·Tracy L Raivio
Sep 9, 2006·FEMS Microbiology Letters·Evan D PepperSteven E Finkel
May 22, 2007·BMC Microbiology·Jintae LeeThomas K Wood
Jan 15, 2008·Journal of Bacteriology·Mary E Laubacher, Sarah E Ades
Jun 10, 2008·Trends in Microbiology·Anindya S GhoshDavid E Nelson
Aug 22, 2009·Microbiology·Lionel FerrièresDavid J Clarke
Sep 19, 2009·PLoS Genetics·Stéphanie Bury-MonéPhilippe Bouloc
May 18, 2010·Microbial Pathogenesis·Hidetada HirakawaKunihiko Nishino
Sep 28, 2010·Research in Microbiology·Dorota Kuczyńska-WiśnikEwa Laskowska
Dec 25, 2010·Cell·Athanasios TypasWaldemar Vollmer
Dec 25, 2010·Cell·Catherine Paradis-BleauThomas G Bernhardt
Oct 1, 2010·Indian Journal of Microbiology·Mingxi HuYongjun Feng
Citations
Oct 9, 2014·Current Microbiology·Pranab K HalderAnindya S Ghosh
Aug 3, 2013·Current Microbiology·Dhriti MallikAnindya S Ghosh
Jul 19, 2015·FEMS Microbiology Letters·Akash KumarAnindya S Ghosh
Dec 1, 2015·Biotechnology and Bioengineering·Lili FengZhiwen Wang
Aug 16, 2016·Journal of Proteomics·Isabelle PoirierMartine Bertrand
Mar 23, 2018·FEMS Microbiology Letters·Dhriti MallikAnindya S Ghosh
Mar 13, 2014·Infection and Immunity·Tobias DörrMatthew K Waldor
Aug 20, 2014·Antimicrobial Agents and Chemotherapy·Uyen T NguyenLori L Burrows
Jan 8, 2014·The Journal of Biological Chemistry·Emilisa FrirdichErin C Gaynor
Feb 11, 2020·FEMS Microbiology Letters·Jianhua YinZhiliang Yu
Dec 14, 2016·Antimicrobial Agents and Chemotherapy·Kristin R BakerLuca Guardabassi
Feb 20, 2019·Frontiers in Microbiology·Jan NevermannJuan A Fuentes
Jan 18, 2017·Journal of Bacteriology·Dev K RanjitKevin D Young
Jul 6, 2019·Frontiers in Microbiology·Weiwei HuangYanbing Ma
Oct 6, 2020·Frontiers in Microbiology·Yuying FuXiangmin Lin
Jan 5, 2021·Frontiers in Microbiology·Wen-Chun HuangChing-Hao Teng
Feb 5, 2021·Microbiology·Sathi MallickAnindya S Ghosh
Mar 31, 2021·Environmental Science and Pollution Research International·Monika SharmaRup Lal
Apr 19, 2018·ACS Synthetic Biology·Wen GaoXiangdong Gao
Oct 9, 2021·Annual Review of Microbiology·Laura AlvarezFelipe Cava
Nov 17, 2021·PloS One·Andrea Olga PapadopoulosBavesh Davandra Kana