Deletion of the cytoplasmic tail of the fusion protein of the paramyxovirus simian virus 5 affects fusion pore enlargement

Journal of Virology
R E Dutch, Robert A Lamb

Abstract

The fusion (F) protein of the paramxyovirus simian parainfluenza virus 5 (SV5) promotes virus-cell and cell-cell membrane fusion. Previous work had indicated that removal of the SV5 F protein cytoplasmic tail (F Tail- or FDelta19) caused a block in fusion promotion at the hemifusion stage. Further examination has shown that although the F Tail- mutant is severely debilitated in promotion of fusion as measured by using two reporter gene assays and is debilitated in the formation of syncytia relative to the wild-type F protein, the F Tail- mutant is capable of promoting the transfer of small aqueous dyes. These data indicate that F Tail- is fully capable of promoting formation of small fusion pores. However, enlargement of fusion pores is debilitated, suggesting that either the cytoplasmic tail of the F protein plays a direct role in pore expansion or that it interacts with other components which control pore growth.

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Citations

Feb 24, 2006·Molecular Biology of the Cell·Scott NolanEric Grote
Jan 19, 2007·Molecular Biology of the Cell·Tamar GattegnoBenjamin Podbilewicz
Jun 18, 2011·Journal of Virology·Eva MittlerStephan Becker
Jul 29, 2004·Journal of Virology·Shaguna SethRichard W Compans
Sep 6, 2005·Journal of Virology·Homer PantuaTrudy G Morrison
Nov 16, 2010·Virology Journal·Dimple Khetawat, Christopher C Broder
Feb 20, 2007·PLoS Pathogens·Karin StiasnyFranz X Heinz
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Jul 31, 2012·Chemical Physics·Ayanjeet Ghosh, Robin M Hochstrasser
Jul 21, 2004·Current Opinion in Cell Biology·Thomas H Söllner
Jun 11, 2015·European Journal of Cell Biology·Michael Weis, Andrea Maisner
Jan 23, 2004·Nature·Yorgo ModisStephen C Harrison
Oct 25, 2007·Proceedings. Biological Sciences·Alex J McCarthySimon J Goodman
Jan 19, 2008·Avian Pathology : Journal of the W.V.P.A·K M ZaffutoC L Afonso
Feb 1, 2017·Current Clinical Microbiology Reports·Hector C AguilarGunner P Johnston
May 17, 2012·Viruses·Andres Chang, Rebecca E Dutch
Jul 23, 2003·Biochimica Et Biophysica Acta·Trudy G Morrison
Nov 17, 2021·Biochemical Society Transactions·Alzbeta Tuerkova, Peter M Kasson

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