Deletion of the genes encoding the type III secretion system and cytotoxic enterotoxin alters host responses to Aeromonas hydrophila infection

Microbial Pathogenesis
Amin A FadlAshok K Chopra

Abstract

In our previous study, we deleted the gene encoding Aeromonas outer membrane protein B (AopB), a structural component of the type III secretion system (T3SS) from a cytotoxic enterotoxin gene (act)-minus diarrheal isolate SSU of Aeromonas hydrophila. Our laboratory also molecularly characterized the cytotoxic enterotoxin (Act), which is secreted by the bacterium utilizing the type II secretion system (T2SS). The act/aopB mutant exhibited significantly reduced cytotoxicity to cultured cells (e.g. RAW 264.7 murine macrophages and HT-29 human colonic epithelial cells) and was avirulent in mice. In this study, we developed additional A. hydrophila mutants in which T3SS-associated ascV and acrV genes were deleted, either individually or in combination with that of the act gene, to examine host-pathogen interactions. A significant reduction in the induction of inflammatory cytokines and chemokines was noted in the sera of mice infected with these mutants when compared to animals infected with wild-type (WT) A. hydrophila. After infection with the WT and act/aopB mutant, we performed microarray analyses on RNA from the above-mentioned murine macrophages and human colonic epithelial cells to examine global cellular transcriptional resp...Continue Reading

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Citations

Sep 4, 2013·Applied Microbiology and Biotechnology·Xue GaoGuo-Qiang Chen
Mar 18, 2008·Journal of Bacteriology·Tatiana E ErovaAshok K Chopra
Sep 19, 2006·Journal of Bacteriology·Rekha SeshadriJohn F Heidelberg
Oct 31, 2012·International Journal of Food Microbiology·Maria João CarvalhoMaria José Saavedra
Oct 15, 2013·Microbial Biotechnology·Philippe Vanden Bergh, Joachim Frey
Sep 10, 2016·Frontiers in Microbiology·Cody R Rasmussen-IveyMark R Liles

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