PMID: 7545284Aug 25, 1995Paper

Deletions at short direct repeats and base substitutions are characteristic mutations for bleomycin-induced double- and single-strand breaks, respectively, in a human shuttle vector system

Nucleic Acids Research
M E Dar, T J Jorgensen

Abstract

Using the radiomimetic drug, bleomycin, we have determined the mutagenic potential of DNA strand breaks in the shuttle vector pZ189 in human fibroblasts. The bleomycin treatment conditions used produce strand breaks with 3'-phosphoglycolate termini as > 95% of the detectable dose-dependent lesions. Breaks with this end group represent 50% of the strand break damage produced by ionizing radiation. We report that such strand breaks are mutagenic lesions. The type of mutation produced is largely determined by the type of strand break on the plasmid (i.e. single versus double). Mutagenesis studies with purified DNA forms showed that nicked plasmids (i.e. those containing single-strand breaks) predominantly produce base substitutions, the majority of which are multiples, which presumably originate from error-prone polymerase activity at strand break sites. In contrast, repair of linear plasmids (i.e. those containing double-strand breaks) mainly results in deletions at short direct repeat sequences, indicating the involvement of illegitimate recombination. The data characterize the nature of mutations produced by single- and double-strand breaks in human cells, and suggests that deletions at direct repeats may be a 'signature' mutat...Continue Reading

References

May 25, 1992·Nucleic Acids Research·T A WintersT J Jorgensen
Oct 20, 1992·Journal of Molecular Biology·A GentilA Sarasin
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R J Steighner, L F Povirk
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S Canning, T P Dryja
Apr 15, 1986·Biochemical Pharmacology·J DecuyperA Van de Vorst
Jul 11, 1988·Nucleic Acids Research·W J DowerC W Ragsdale
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A BredbergM M Seidman
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M M SeidmanK H Kraemer
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
May 1, 1993·Mutation Research·L H MullendersA A van Zeeland
Sep 1, 1994·Molecular and Cellular Biology·J W Phillips, W F Morgan
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T Morris, J Thacker

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Citations

May 8, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K W Caldecott
Feb 22, 2001·Mutation Research·G TellG Damante
Dec 29, 2009·The Journal of Biological Chemistry·Mustapha AouidaDindial Ramotar
Sep 14, 2001·Antioxidants & Redox Signaling·M R Kelley, S H Parsons
Dec 15, 1996·Nucleic Acids Research·R M MasonM P Fairman
Aug 4, 2005·Nucleic Acids Research·Valeria SimonelliPaola Fortini
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·P WangL F Povirk
Jun 1, 1996·International Journal of Radiation Biology·N CheongG Iliakis
Feb 23, 2013·Mechanisms of Ageing and Development·Kimberly D JacobMichele K Evans
Oct 2, 2002·Environmental and Molecular Mutagenesis·Alisson Marques de Miranda Cabral GontijoJoão Lauro Viana de Camargo
Dec 30, 2015·Antimicrobial Agents and Chemotherapy·Lisa Yun SongJeffrey H Miller
Jan 31, 2003·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Hujie Wang, Dindial Ramotar

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