PMID: 15218950Jun 29, 2004Paper

Deletions of chromosomes 1p and 19q are detectable on frozen smears of gliomas by FISH: usefulness for stereotactic biopsies

Journal of Neuro-oncology
C BouvierD Figarella-Branger

Abstract

Among diffuse gliomas, oligodendrogliomas may account for 25% of cases. They have a better prognosis and chemosensitivity as compared to astrocytomas. Genetic studies have shown a correlation between oligodendrocyte phenotype and presence of 1p/19q deletions. In addition, these deletions are of prognostic value. The aim of the present study was to describe a new method to detect 1p/19q deletions when little tumoral material is available (stereotactic biopsies (SBs)). Since smears (cytological preparations) are routinely done for intraoperative diagnosis of gliomas, we have searched for 1p/19q deletions by FISH in a series of 30 patients with a glioma. In 14 cases, loss of heterozygosity (LOH) analysis was also performed in order to validate our method. We found that FISH analysis on frozen smears was a simple, rapid and reliable method to detect 1p/19q deletions and a good concordance was found with LOH data (85%). The main advantages of FISH analysis on frozen smears are the following. First, it requires little material and can be easily done in the case of SBs. Second, it has a higher sensitivity than LOH especially in infiltrative areas of gliomas. Third, it allows detection of a codeletion 1p/19q in a single tumor cell. In ...Continue Reading

Citations

Apr 8, 2006·Neuropathology and Applied Neurobiology·C ColinD Figarella-Branger
Oct 30, 2009·American Journal of Clinical Pathology·Dipti Mahajan, Richard A Prayson
Apr 20, 2007·Neuropathology and Applied Neurobiology·C ColinD Figarella-Branger
Feb 3, 2006·Acta Neuropathologica·Marc-Antoine Belaud-RotureauJean-Philippe Merlio
Aug 15, 2012·Journal of Neuro-oncology·Dominique Figarella-BrangerPhilippe Metellus
Jul 14, 2016·Journal of Neuro-oncology·Irena Srebotnik-Kirbiš, Clara Limbäck-Stokin
Sep 10, 2009·Cancer Genetics and Cytogenetics·Macoura GadjiRégen Drouin

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