Delineating the role of polyphenol oxidase in the darkening of alkaline wheat noodles

Journal of Agricultural and Food Chemistry
E P FuerstCraig F Morris

Abstract

This study evaluated the effects of inhibitors on polyphenol oxidase (PPO) activity, the effect of the PPO inhibitor tropolone on noodle darkening, and the correlation of PPO activity with darkening of alkaline noodles. The PPO inhibitors tropolone and salicylhydroxamic acid (each at 1 microM) reduced kernel PPO activity by approximately 50% in three hexaploid wheat cultivars but did not inhibit PPO activity in the two very low PPO cultivars, durum Langdon, and the synthetic hexaploid-derived ID580. Tropolone (100 microg/g flour) inhibited alkaline noodle darkening (deltaL*) by 13-25% in the low PPO wheat cultivar, ID377s, and by 39-54% in the high PPO wheat cultivar, Klasic. Alkaline noodle darkening among 502 wheat samples was correlated with kernel PPO activity (r = 0.64). Results substantiate the hypothesis that PPO plays a major role in darkening of alkaline noodles. However, results also indicate that substantial darkening would occur even at zero PPO activity, as measured in the kernel PPO assay. Therefore, darkening of alkaline noodles is probably due to the cultivar-specific level of PPO activity and the presence of at least one additional darkening mechanism. Further investigation is required to identify the phenolic ...Continue Reading

References

Mar 22, 2000·Journal of Agricultural and Food Chemistry·M Pérez-Gilabert, F García Carmona
Dec 16, 2000·Phytochemistry·P Mazzafera, S P Robinson

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Citations

Jul 17, 2009·Plant Physiology·Daniela WahlerDirk Prüfer
Nov 26, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Shengnan ZhaiXianchun Xia
Feb 8, 2018·Frontiers in Plant Science·E Patrick FuerstPatricia A Okubara
May 18, 2018·Journal of the Science of Food and Agriculture·Craig F Morris
Jul 1, 2014·Comprehensive Reviews in Food Science and Food Safety·Man LiHui-Ming Zhou
May 28, 2009·Journal of Agricultural and Food Chemistry·Robert E AsenstorferDaryl J Mares
Mar 20, 2010·Journal of Agricultural and Food Chemistry·Robert E AsenstorferDaryl J Mares
Nov 8, 2018·Journal of Agricultural and Food Chemistry·Linda BrütschSimon Kuster

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