Delineation by use of specific monoclonal antibodies of the T-cell receptor and major histocompatibility complex interaction sites on the superantigen toxic shock syndrome toxin 1.

Infection and Immunity
R ShimonkevitzM Matsumura

Abstract

Murine monoclonal antibodies (MAbs) specific for toxic shock syndrome toxin 1 (TSST-1), a bacterial superantigen, showed the ability either to detect TSST-1 bound to histocompatibility locus antigen (HLA)-DR molecules or to inhibit TSST-1 binding to HLA-DR. A MAb capable of detecting DR-bound TSST-1 could also inhibit the toxin-induced activation of a T-cell receptor Vbeta15-expressing murine T-cell hybridoma. Alternatively, MAbs with specificity for the HLA-DR association site could present TSST-1 in vitro, stimulating CD4+ human T cells to proliferate. These functional activities correlated directly with with MAb specificity for HLA-DR versus T-cell receptor Vbeta interaction sites on TSST-1 as determined by reactivity with a panel of recombinant TSST-1 mutant molecules. Therefore, these MAbs discriminate the superantigen functional sites on the TSST-1 molecule and constitute reagents with the property of being potent modulators of the toxic activity of TSST-1.

References

Feb 1, 1992·The Journal of Experimental Medicine·J W KapplerP Marrack
Jan 1, 1990·Critical Reviews in Microbiology·G A BohachP M Schlievert
Oct 1, 1988·Clinical Microbiology Reviews·J K Todd
Aug 1, 1994·The Journal of Experimental Medicine·A R HamadJ W Kappler
Nov 1, 1994·Biochemistry·R L DeresiewiczD L Kasper
Mar 1, 1993·The Journal of Experimental Medicine·J AbeD Y Leung
Dec 21, 1993·Biochemistry·G S PrasadD H Ohlendorf
Jan 1, 1993·Advances in Immunology·B L KotzinP Marrack

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