Delineation of the endemic and sporadic methicillin-resistant Staphylococcus aureus clones in a Czech hospital

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
O MelterH de Lencastre

Abstract

The aim of this study was to define the endemic clones of methicillin-resistant Staphylococcus aureus (MRSA) among strains collected between September, 2001, and February, 2003, at the regional hospital of Nový Jicín, Czech Republic. The isolates were characterized by susceptibility tests, HindIII ribotyping, and pulsed-field gel electrophoresis. Representatives of each clonal type were analyzed by multilocus sequence typing and staphylococcal cassette chromosome mec (SCCmec) typing. The prevalence of the most important macrolide (ermA, ermB, ermC, and msrA) and aminoglycoside (aac6'-aph2", aph3', and ant4') resistance genes was evaluated as well. Our results document the existence of two international MRSA clones: (1) the Iberian clone (ST247:SCCmec IA:PFGE A:ribotype H2), endemic in the hospital and associated to a single multiresistant phenotype; and (2) clone EMRSA-15 (ST22:SCCmec IV:PFGE H-ribotype H7), appearing in the beginning of 2002 and associated with three phenotypes. These two clones could be distinguished by antibiogram, distribution of macrolide and aminoglycoside resistance genes (ermA, aac6'-aph2", ant4' versus ermC and msrA in a few isolates), production of beta-lactamase, and presence of enterotoxin A (in the...Continue Reading

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Citations

Dec 13, 2005·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·E E UdoP W J West
Feb 8, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·G Echániz-AvilesH de Lencastre
Dec 12, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·K KrziwanekH Mittermayer
Feb 3, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E E UdoB Noronha
Feb 23, 2007·Journal of Medical Microbiology·Li-Yang HsuTse-Hsien Koh
May 17, 2012·Folia Microbiologica·P MelicherčíkA Sosna

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