Delipidation of Plasma Has Minimal Effects on Human Butyrylcholinesterase

Frontiers in Pharmacology
Seda OnderOksana Lockridge

Abstract

Human butyrylcholinesterase (BChE) is purified in large quantities from Cohn fraction IV-4 to use for protection against the toxicity of chemical warfare agents. Small scale preliminary experiments use outdated plasma from the American Red Cross as the starting material for purifying BChE (P06276). Many of the volunteer donor plasma samples are turbid with fat, the donor having eaten fatty food before the blood draw. The turbid fat interferes with enzyme assays performed in the spectrophotometer and with column chromatography. Our goal was to find a method to remove fat from plasma without loss of BChE activity. Satisfactory delipidation was achieved by adding a solution of 10% dextran sulfate and calcium chloride to fatty plasma, followed by centrifugation, and filtration through a 0.8 μm filter. Treatment with Aerosil also delipidated fatty plasma, but was accompanied by loss of 50% of the plasma volume. BChE activity and the BChE isozyme pattern on nondenaturing gel electrophoresis were unaffected by delipidation. BChE in delipidated plasma was efficiently captured by immobilized monoclonal antibodies B2 18-5 and mAb2. The immunopurified BChE was released from antibody binding with acid and visualized as a highly enriched, d...Continue Reading

References

Nov 26, 1987·Biochimica Et Biophysica Acta·A Ferro, P Masson
Jun 1, 1965·Immunochemistry·R MasseyeffJ Josselin
Apr 1, 1983·Clinical Biochemistry·S T AgneseW H Hannon
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE
Mar 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M J KARNOVSKY, L ROOTS
Mar 18, 2004·Journal of Biochemical and Biophysical Methods·T P L FerrazN M Soares
Jul 31, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yue-Jin HuangSolomon Langermann
Dec 6, 2011·Biochemia Medica·Pilar Calmarza, José Cordero
Apr 16, 2013·BioMed Research International·Yanli WangEnqi Liu
Mar 15, 2014·Biochemia Medica·Nora Nikolac
Sep 30, 2014·Biochemical Pharmacology·Herve DelacourOksana Lockridge

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Citations

Jul 28, 2020·3 Biotech·Achintya Kumar Dolui, Panneerselvam Vijayaraj

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Datasets Mentioned

BETA
KT189143
KT189144
KJ141199
KJ141200

Methods Mentioned

BETA
column chromatography
electrophoresis
blood draw

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