Delivering Single-Walled Carbon Nanotubes to the Nucleus Using Engineered Nuclear Protein Domains

ACS Applied Materials & Interfaces
Patrick D BoyerKris Noel Dahl

Abstract

Single-walled carbon nanotubes (SWCNTs) have great potential for cell-based therapies due to their unique intrinsic optical and physical characteristics. Consequently, broad classes of dispersants have been identified that individually suspend SWCNTs in water and cell media in addition to reducing nanotube toxicity to cells. Unambiguous control and verification of the localization and distribution of SWCNTs within cells, particularly to the nucleus, is needed to advance subcellular technologies utilizing nanotubes. Here we report delivery of SWCNTs to the nucleus by noncovalently attaching the tail domain of the nuclear protein lamin B1 (LB1), which we engineer from the full-length LMNB1 cDNA. More than half of this low molecular weight globular protein is intrinsically disordered but has an immunoglobulin-fold composed of a central hydrophobic core, which is highly suitable for associating with SWCNTs, stably suspending SWCNTs in water and cell media. In addition, LB1 has an exposed nuclear localization sequence to promote active nuclear import of SWCNTs. These SWCNTs-LB1 dispersions in water and cell media display near-infrared (NIR) absorption spectra with sharp van Hove peaks and an NIR fluorescence spectra, suggesting that...Continue Reading

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Citations

Mar 17, 2017·ACS Applied Materials & Interfaces·Alessandra AntonucciArdemis A Boghossian
Mar 25, 2017·Nanomedicine·Moumita RayVincent M Rotello
Jan 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Brian D HoltKris Noel Dahl
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Aug 22, 2017·Advanced Functional Materials·Haoyuan Huang, Jonathan F Lovell
Sep 10, 2019·Advanced Materials·Xiaofei QinWei Huang
Feb 24, 2017·The Journal of Chemical Physics·Danilo RoccatanoRonen Zangi
Mar 2, 2021·Advanced Healthcare Materials·Brian D HoltStefanie A Sydlik
Oct 31, 2018·Open Life Sciences·Agnieszka Pudlarz, Janusz Szemraj
Oct 3, 2018·ACS Applied Materials & Interfaces·Shang-Jung WuArdemis A Boghossian
Oct 27, 2017·Bioconjugate Chemistry·Edson José ComparettiRamon Kaneno
Oct 20, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Ricardo M F FernandesIstván Furó
May 4, 2017·Acta Biomaterialia·Adem GuvenMichael T Lewis

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