Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair

Scientific Reports
Ivana GrulovaD Cizkova

Abstract

Spinal cord injury (SCI) has been implicated in neural cell loss and consequently functional motor and sensory impairment. In this study, we propose an alginate-based neurobridge enriched with/without trophic growth factors (GFs) that can be utilized as a therapeutic approach for spinal cord repair. The bioavailability of key GFs, such as Epidermal Growth factor (EGF) and basic Fibroblast Growth Factor (bFGF) released from injected alginate biomaterial to the central lesion site significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons (choline acetyltransferase positive motoneurons) and sensory fibres. In addition, we document enhanced outgrowth of corticospinal tract axons and presence of blood vessels at the central lesion. Tissue proteomics was performed at 3, 7 and 10 days after SCI in rats indicated the presence of anti-inflammatory factors in segments above the central lesion site, whereas in segments below, neurite outgrowth factors, inflammatory cytokines and chondroitin sulfate proteoglycan of the lectican protein family were overexpressed. Collectively, based on our data, we confirm that functional recovery was significantly improved in SCI groups receiving alginate scaffo...Continue Reading

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Citations

Sep 23, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Kwang-Hsiao SunHuan Zhang
May 10, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Genlong JiaoRui Guo
Mar 4, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Genlong JiaoZhizhong Li
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May 31, 2019·Micromachines·Alena SergeevaDmitry Volodkin
Apr 25, 2020·Molecular Neurobiology·Hadi Abou-El-HassanIbrahim Omeis
Jan 30, 2018·Frontiers in Cellular Neuroscience·Shengwen LiuRadhika Puttagunta
Apr 30, 2019·Frontiers in Molecular Neuroscience·Xizhi WangXuehong Liu
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Jul 24, 2021·Frontiers in Cell and Developmental Biology·Eva SykovaSarka Kubinova

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Methods Mentioned

BETA
deamination
acetylation

Software Mentioned

Thermo Scientific Proteome Discoverer
MaxQuant
SEQUEST®
Andromeda
Perseus
Graph pad PRISM
Image J
ImageJ
Decoy

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