PMID: 9181456May 1, 1997Paper

Delivery of cytokines by liposomes. III. Liposome-encapsulated GM-CSF and TNF-alpha show improved pharmacokinetics and biological activity and reduced toxicity in mice

Journal of Immunotherapy
E KedarY Barenholz

Abstract

In an attempt to potentiate the therapeutic index of cytokines, recombinant mouse granulocyte/macrophage colony-stimulating factor (GM-CSF) and recombinant human tumor necrosis factor alpha (TNF-alpha) were encapsulated in large (0.3-2.2 microns) multilamellar vesicles composed of various lipids, using several encapsulation methods. Liposomal cytokine activity was tested in vitro and in vivo and was compared with that of the soluble cytokines. The main observations were as follows: (a) The mean encapsulation efficiency, as determined by bioassays, was 49-79%, depending on the formulation, for GM-CSF, and 48% for TNF-alpha; (b) some of the entrapped cytokine preparations displayed high stability at 4 degrees C, with < 30% loss of biologic activity during a 4-month period; (c) release of TNF-alpha, but not of GM-CSF, from the liposomes was required for their biological activity in vitro; (d) plasma half-lives (t1/2 alpha, t1/2 beta) and the area under the curve (AUC) of the entrapped cytokines were 10-20 times greater than those of the soluble cytokines; (e) the toxicity of liposomal TNF-alpha was one-third and one-seventh that of soluble TNF-alpha in normal and tumor-bearing mice, respectively; (f) administration of liposomal GM...Continue Reading

Citations

Jan 20, 2011·Bulletin of Experimental Biology and Medicine·S G GamaleyV I Masycheva
Mar 29, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·Y LiX Gao
Apr 11, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·Y P LiX J Gao
Nov 5, 2002·Critical Reviews in Oncology/hematology·A H KlimpT Daemen
Oct 1, 1998·Cytokine & Growth Factor Reviews·R Kaempfer
Feb 12, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Y LiuP L Hermonat
Jul 31, 2009·Journal of Biomechanical Engineering·Raghav GoelJohn C Bischof
Nov 12, 2009·Arthritis Research & Therapy·Fons A van de Loo, Wim B van den Berg
Feb 16, 2012·International Journal of Nanomedicine·Abolfazl AkbarzadehSoodabeh Davaran
Apr 20, 2012·Immunotherapy·David A Christian, Christopher A Hunter
Dec 4, 2013·Expert Opinion on Drug Delivery·Lisa MullenYuti Chernajovsky
Jul 16, 2014·Artificial Cells, Nanomedicine, and Biotechnology·Sedigheh Fekri AvalNosratollah Zarghami
Jan 13, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Soumaye AmirsaadatNosratollah Zarghami
Jun 5, 2003·Technology in Cancer Research & Treatment·Timo L M ten Hagen, A M M Eggermont
May 31, 2017·Advanced Healthcare Materials·Arne KienzleHelmut Jonuleit
May 3, 2003·Journal of Liposome Research·Yechezkel Barenholz
May 18, 2016·Experimental Biology and Medicine·Sourabh Shukla, Nicole F Steinmetz
Aug 26, 1998·International Journal of Cancer. Journal International Du Cancer·A H van der VeenT L ten Hagen
May 2, 2006·Molecular Cancer Therapeutics·Rachana K VisariaJohn C Bischof

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