PMID: 11340139May 8, 2001Paper

Delivery of endothelial cells to balloon-dilated rabbit arteries with use of a local delivery catheter

Journal of Vascular and Interventional Radiology : JVIR
E DillavouP M Consigny

Abstract

Experiments were performed to determine if a local delivery catheter could deliver endothelial cells that would be retained on the luminal surface of balloon-dilated arteries. Six New Zealand White rabbits underwent carotid catheterization, arteriography, and balloon angioplasty of an external iliac artery. A local delivery catheter (Dispatch) was then positioned at the site of angioplasty and the 3-mm balloon was inflated. Cultured rabbit endothelial cells (1.26 +/- 0.3 x 10(6) ), previously stained with fluorescent dye PKH26, were delivered to the artery in three infusions separated by 10 minutes. The delivery balloon was deflated and removed 25 minutes after the last delivery. The arteries were then perfusion-fixed in situ at physiologic pressure, removed, and divided into four segments, and the segments were rapidly frozen and cryosectioned. Eight sections from each arterial segment were examined by means of epifluorescence microscopy. The luminal surface of each artery was visually divided into eight sectors of equal length and each sector was assigned a score based on the degree of endothelial coverage (0 = no coverage, 1 = <50% coverage, 2 = >50% coverage). The endothelial coverage score for the six arteries averaged 0.4...Continue Reading

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T MaciagR Forand
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T D BjornssonK A Thomas
Mar 1, 1989·Radiology·G J BeckerM D Dake
Jun 1, 1983·The Journal of Clinical Investigation·P A KernR H Eckel
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·A NathanE R Edelman
Mar 1, 1993·European Journal of Vascular Surgery·M M ThompsonP R Bell
Mar 21, 1998·The Journal of Clinical Investigation·R D RudicW C Sessa
Jun 9, 1998·Journal of Vascular and Interventional Radiology : JVIR·P M Consigny, N J Vitali
Mar 6, 1999·Circulation Research·H M NugentE R Edelman

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