Abstract
Experiments were performed to determine if a local delivery catheter could deliver endothelial cells that would be retained on the luminal surface of balloon-dilated arteries. Six New Zealand White rabbits underwent carotid catheterization, arteriography, and balloon angioplasty of an external iliac artery. A local delivery catheter (Dispatch) was then positioned at the site of angioplasty and the 3-mm balloon was inflated. Cultured rabbit endothelial cells (1.26 +/- 0.3 x 10(6) ), previously stained with fluorescent dye PKH26, were delivered to the artery in three infusions separated by 10 minutes. The delivery balloon was deflated and removed 25 minutes after the last delivery. The arteries were then perfusion-fixed in situ at physiologic pressure, removed, and divided into four segments, and the segments were rapidly frozen and cryosectioned. Eight sections from each arterial segment were examined by means of epifluorescence microscopy. The luminal surface of each artery was visually divided into eight sectors of equal length and each sector was assigned a score based on the degree of endothelial coverage (0 = no coverage, 1 = <50% coverage, 2 = >50% coverage). The endothelial coverage score for the six arteries averaged 0.4...Continue Reading
References
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T MaciagR Forand
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T D BjornssonK A Thomas
Jun 16, 1989·Science·E G NabelG J Nabel
Mar 1, 1989·Radiology·G J BeckerM D Dake
Feb 1, 1988·Circulation·P W SerruysP G Hugenholtz
Jan 1, 1987·Journal of Vascular Surgery·H L BushD Deykin
Jun 1, 1983·The Journal of Clinical Investigation·P A KernR H Eckel
Nov 1, 1995·Circulation·E CamenzindP W Serruys
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·A NathanE R Edelman
Jun 1, 1995·Circulation·T AsaharaJ M Isner
Feb 1, 1995·Circulation·J F MitchelR G McKay
Mar 1, 1994·Journal of Vascular Surgery·M M ThompsonP R Bell
May 1, 1994·Circulation·M S ConteR C Mulligan
Jun 1, 1994·Circulation·T KakutaD P Faxon
Mar 1, 1993·European Journal of Vascular Surgery·M M ThompsonP R Bell
Jul 1, 1996·Circulation·G S MintzM B Leon
Mar 21, 1998·The Journal of Clinical Investigation·R D RudicW C Sessa
Jun 9, 1998·Journal of Vascular and Interventional Radiology : JVIR·P M Consigny, N J Vitali
Mar 6, 1999·Circulation Research·H M NugentE R Edelman
Mar 19, 1999·Journal of Vascular and Interventional Radiology : JVIR·P M ConsignyN J Vitali
Jul 28, 1999·Coronary Artery Disease·L Y YangD R Abendschein