Delivery of iron-sulfur clusters to the hydrogen-oxidizing [NiFe]-hydrogenases in Escherichia coli requires the A-type carrier proteins ErpA and IscA.

PloS One
Constanze Pinske, R Gary Sawers

Abstract

During anaerobic growth Escherichia coli synthesizes two membrane-associated hydrogen-oxidizing [NiFe]-hydrogenases, termed hydrogenase 1 and hydrogenase 2. Each enzyme comprises a catalytic subunit containing the [NiFe] cofactor, an electron-transferring small subunit with a particular complement of [Fe-S] (iron-sulfur) clusters and a membrane-anchor subunit. How the [Fe-S] clusters are delivered to the small subunit of these enzymes is unclear. A-type carrier (ATC) proteins of the Isc (iron-sulfur-cluster) and Suf (sulfur mobilization) [Fe-S] cluster biogenesis pathways are proposed to traffic pre-formed [Fe-S] clusters to apoprotein targets. Mutants that could not synthesize SufA had active hydrogenase 1 and hydrogenase 2 enzymes, thus demonstrating that the Suf machinery is not required for hydrogenase maturation. In contrast, mutants devoid of the IscA, ErpA or IscU proteins of the Isc machinery had no detectable hydrogenase 1 or 2 activities. Lack of activity of both enzymes correlated with the absence of the respective [Fe-S]-cluster-containing small subunit, which was apparently rapidly degraded. During biosynthesis the hydrogenase large subunits receive their [NiFe] cofactor from the Hyp maturation machinery. Subsequen...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Oct 1, 1989·Molecular & General Genetics : MGG·M R JacobsonD R Dean
Aug 1, 1985·Journal of Bacteriology·S P Ballantine, D H Boxer
Apr 1, 1967·Analytical Biochemistry·J R Loewenberg
Jan 1, 1994·Antonie van Leeuwenhoek·G Sawers
Feb 16, 1995·Nature·A VolbedaJ C Fontecilla-Camps
Jan 27, 2000·Archives of Microbiology·T Eitinger, M A Mandrand-Berthelot
Jun 29, 2002·The Journal of Biological Chemistry·Yasuhiro Takahashi, Umechiyo Tokumoto
Oct 16, 2002·The Journal of Biological Chemistry·Athanasios PaschosAugust Böck
Jul 28, 2004·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·S Ollagnier-de-ChoudensM Fontecave
Sep 24, 2004·The EMBO Journal·Rachael L JackFrank Sargent
Jun 15, 2005·Annual Review of Biochemistry·Deborah C JohnsonMichael K Johnson
Jul 4, 2006·FEBS Letters·Melanie Blokesch, August Böck
Aug 1, 2006·The Journal of Biological Chemistry·Juanjuan YangHuangen Ding
Nov 10, 2006·Advances in Microbial Physiology·August BöckMatthew C Posewitz
Jan 12, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Lucia Forzi, R Gary Sawers
Aug 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Laurent LoiseauFrédéric Barras
Oct 12, 2007·Chemical Reviews·Paulette M Vignais, Bernard Billoud
Mar 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Carla Ayala-CastroF Wayne Outten
Oct 9, 2009·Biochemistry·Harsimranjit K ChahalF Wayne Outten
May 15, 2010·Nature Reviews. Microbiology·Béatrice Py, Frédéric Barras

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Citations

Mar 25, 2014·Chemical Reviews·Wolfgang LubitzEdward Reijerse
Jun 28, 2012·Biochemical and Biophysical Research Communications·Basem SobohR Gary Sawers
Dec 17, 2014·Biochimica Et Biophysica Acta·B BlancS Ollagnier de Choudens
May 11, 2013·Biochimica Et Biophysica Acta·Béatrice RocheFrédéric Barras
Jan 10, 2013·Biochimica Et Biophysica Acta·Béatrice RocheFrédéric Barras
Jan 27, 2015·Metallomics : Integrated Biometal Science·Constanze PinskeR Gary Sawers
Jul 19, 2018·Metallomics : Integrated Biometal Science·Y BaiA Sawyer
Jan 17, 2013·Molecular Microbiology·Daniel VinellaFrédéric Barras
Aug 28, 2016·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Lisa BowmanAlison Parkin
Aug 29, 2019·The Journal of Biological Chemistry·Michael J LacasseDeborah B Zamble

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Methods Mentioned

BETA
electrophoresis

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